摘要
目的:建立HPLC法测定环磷腺苷葡胺注射液中环磷腺苷的含量。方法:采用HypersilC_(18)柱(250 mm×4.6 mm,5μm),流动相为0.01%磷酸溶液-乙腈(95:5),流速为1.0 ml·min^(-1),检测波长为259 nm,柱温为30℃。结果:环磷腺苷在0.051～2.054μg范围内线性关系良好,r=1.000 0(n=6),平均回收率为99.61%(n=9),RSD为0.80%。结论:该方法灵敏、准确、专属性强,能简便有效控制环磷腺苷葡胺注射液中环磷腺苷的含量。
Objective： To establish an HPLC method for the determination of cAMP in meglumine adenosine cyciophosphate injection. Method： Hypersil C18 column （250 mm ×4. 6 mm, 5μm） set at 30 ℃ was used . The mobile phase was 0. 01% H3PO4 solution and acetonitrile （95：5） whb flow rate of 1.0 ml·min ^-1, and the detection wavelength was set at 259 nm. Result： A good linearity was obtained over the range of 0. 051-2. 054 lag（r = 1. 000 0, n = 6）. The average recovery was 99. 61% （RSD = 0. 80%, n = 9）. Conclusion： The method is sensitive, accurate and specific,and can be used for the quality eontrol of meglumine adenosine cyelophosphate injection.
出处
《中国药师》
CAS
2011年第5期682-683,共2页
China Pharmacist
