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RASAL1基因在胃癌组织中的表达及临床病理意义 被引量：1

Expression of RASAL1 Gene in Gastric Cancer and Its Clinicopathological Significance
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摘要 [目的]研究RASAL1基因及蛋白在胃癌组织中的表达,并分析其与胃癌临床病理特征的关系,初步探讨RASAL1在胃癌发生发展中的意义。[方法]收集50例胃癌及正常胃组织标本,采用RT-PCR方法检测组织中RASAL1mRNA表达,WesternBlot及免疫组织化学方法检测RASAL1蛋白的表达;并结合胃癌的临床病理资料作相关统计学分析。[结果]①RT-PCR检测显示胃癌组织中RASAL1mRNA表达的平均吸光度值为0.51±0.15,而正常胃组织的平均吸光度值为0.64±0.12,胃癌组织的RASAL1mRNA显著低于正常胃组织(P=0.0004)。②WesternBlot分析表明,胃癌组织RASAL1蛋白表达的平均吸光度值为0.52±0.22,而正常胃组织的平均吸光度值为0.68±0.23,前者显著低于后者(P=0.003)。③免疫组化结果表明,RASAL1蛋白主要在胞浆中表达;50例胃癌组织中RASAL1蛋白的表达分别为12例(-)、23例(+)、13例(++)、2例(+++);而在50例正常胃组织中分别为16例(++)、34例(+++);胃癌组织中RASAL1表达较正常胃组织降低(P<0.05)。④RASAL1蛋白的表达与胃癌患者性别、年龄无显著相关,而与肿瘤大小、分化程度、侵袭深度、淋巴转移及TNM分期相关,随病情进展呈下降趋势。[结论]RASAL1基因在胃癌组织中表达明显下调,且与分化程度及进展阶段相关,提示其可能参与了胃癌的发生发展过程,是胃癌的抑癌基因之一。
作者 张慧 陈洪 杨晴 孔波 杨晓薇 王智 刘洋
机构地区 东南大学附属中大医院
出处 《中国肿瘤》 CAS 2011年第5期378-382,共5页 China Cancer
基金 江苏省自然科学基金(BK2008301)
关键词 RASAL1基因 胃癌 抑癌基因
分类号 R735.2 [医药卫生—肿瘤]
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参考文献16

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同被引文献12

  • 1]in I-I, Wang X, Ying ], et al. Epigenetic silencing of a Ca2 + -regu- lated RAS GTPase-aetivating protein RASAL defines a new mechanism of RAS activation in human cancers[J]. Proc Natl Acad Sci USA, 2007,104(30) : 12353-12358.
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  • 3Bernards A,Settleman J. Loss of the ras regulator RASAL1 : an- other route to ras activation in colorectal cancer[J]. Gastroenter ology,2009,136(1) :46-48.
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  • 5Gy6rffy B, Sch:fer R. Biomarkers downstream o{ RAS: a search for robust transcriptional targets[J]. Curr Cancer Drug Targets, 2010,10(8) : 858-868.
  • 6Chen H,Yang XW, Zhang H,et al. In vivo and in vitro expression of the RASAL1 gene in human gastric adenocareinoma and its clinicopath- ological significance[J]. Oncol Lett,2012,3(3):535-540.
  • 7Qiao F,Su X,Qiu X,et al. Enforced expression of RASAL1 sup- presses cell proliferation and the transformation ability of gastric cancer cells[J]. Oncol Rep,2012,28(4) :1475-1481.
  • 8Ohta M,Seto M,Ijichi H,et al. Decreased expression of the RAS GT- Pase activating protein RASAL1 is associated with colorectal tumor progression[J]. Gastroenterology,2009,136(1) :206 216.
  • 9Aytekin T, Ozaslan M, Cengiz B. Deletion mapping of chromosome region 12q13-24 in colorectal cancer[J]. Cancer Genet Cytogenet, 2010,201(1):32 38.
  • 10张慧,陈洪.Ras基因及其调节异常与肿瘤发生的研究进展[J].中华肿瘤防治杂志,2010,17(12):950-953. 被引量：8

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中国肿瘤
2011年 第5期

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