摘要
[目的]制作游离型腰椎间盘突出动物模型,观察并探讨游离髓核组织重吸收的可能机制,为临床保守治疗某些类型腰椎间盘突出症提供新的思路和依据。[方法]健康雄性新西兰大白兔90只,随机分为三组:模型组60只,假模型组24只,对照组6只。模型组前方入路制作游离型腰椎间盘突出动物模型,假模型组取相同手术入路,但仅暴露至纤维环而不做后续处理,对照组不做任何处理。模型组及假模型组分别于术后2、4、6、8、10、12周分批处死动物,取手术区间椎间盘标本。用免疫组化方法观察髓核组织中CD68+巨噬细胞浸润情况。[结果]模型组移植的髓核组织不同程度缩小甚至消失,所有移植标本内均见CD68+巨噬细胞浸润随时间延长而逐渐减少,假模型组仅纤维环外缘见少量CD68+巨噬细胞浸润,其髓核及对照组髓核均未见CD68+巨噬细胞浸润。[结论](1)运用前方手术入路可建立游离型腰椎间盘突出动物模型;(2)游离髓核组织在硬膜外腔可逐渐被机体重吸收;(3)CD68+巨噬细胞浸润在游离髓核组织重吸收过程中起到重要作用。
[Objective]To observe the possible mechanism of resorption or contraction in the herniated nucleus pulposus（HNP） by making models of lumbar disc herniation（LDH）,so as to provide a new method and basics for conservative treatment of some certain types of LDH tissues.[Methods]Ninety adult and healthy New Zealand rabbits（irrespective of gender） were randomly devided into model group（n=60）,sham operation group（n=24） and control group（n=6）.Sixty rabbits were randomly selected to establish the LDH models.The animals in sham operation group were only exposed to intervertebral discs.Rabbits in the model and sham operation groups were divided into 6 groups,and were sacrificed at 2,4,6,8,10 and 12 weeks after operation.The specimens were observed and analyzed immunohistochemically for the presence of CD68＋ cells.The normal nucleus pulposus were harvested from the animals of the control group and the same immunohistochemical detection was performed.[Results]The various contraction and disappearance of the nucleus pulposus were observed in the model group at different time points after operation.The infiltration of CD68＋ cells presented in the HNP of all the specimens,and the degree of infiltration was decreased as the time prolonged.But there were no CD68＋ cells in the nucleus pulposus of the sham operation and control groups.[Conclusion]（1） The sequestered type of LDH animal model can be established by the anterior surgery.（2） HNP can be absorbed during the epidural space.（3） The CD68＋ cells plays a vital role in the process of HNP＇ s reabsorption.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2011年第9期774-778,共5页
Orthopedic Journal of China
基金
吉林省教育厅科研课题(编号:吉教合字2008-第9号)
关键词
髓核
自然吸收
巨噬细胞
免疫组织化学
nucleus pulpous
spontaneous absorptiosn
macrophage
immunohistochemistry
