摘要
目的体外观察人工合成抗菌肽(synthetic antimicrobial peptide,AMPs)对白色念珠菌的抑制作用。方法人工合成抗菌七肽并制备成含不同药物剂量的药敏纸片,通过纸片扩散法观察AMPs体外对白色念珠菌的抑制作用,测量含不同剂量AMPs的药敏纸片对白色念珠菌产生抑菌环的直径。同时应用杯碟法评价AMPs对白色念珠菌的最小抑菌浓度。结果 AMPs体外可对白色念珠菌产生抑制作用。含AMPs 307.2、153.6、76.8、38.4、19.2、9.6μg的药敏纸片对白色念珠菌产生的抑菌环直径分别为57.89±1.36、37.44±1.24、35.89±1.69、30±5.48、16.44±1.01、10.11±0.60mm,且抑菌环直径与药物剂量间具有正相关性(r=1,P<0.01)。杯碟法显示AMPs对白色念珠菌的最小抑菌浓度为128μg/ml。结论 AMPs对白色念珠菌具有一定抑制作用,此种抑制作用随药物剂量的增加而增强。
Objective To evaluate the inhibiting effects of synthetic antimicrobial peptide（AMPs） on Candida albicans in vitro.Methods The effect of AMPs on Candida albicans was evaluated by K-B method.The inhibition of different dose of AMPs on Candida albibans was also detected by measuring the diameter of the inhibiting zones of Candida albicans.The minimal inhibitory concentration of AMP on Candida albicans was examined by cylinder-plate method.ResultsThe AMPs had significant inhibiting effect on the Candida albicans.The paper with AMPs at concentrations of 307.2,153.6,76.8,38.4,19.2 and 9.6μg lead to the different diameter of inhibiting zone at 57.89±1.36,37.44±1.24,35.89±1.69,30±5.48,16.44±1.01 and 10.11±0.60mm.The diameter of inhibiting zone showed positive correlation with the dose of AMPs（P0.01）.The minimal inhibitory concentration of AMPs on Candida albicans was 128μg/ ml.Conclusion AMPs had inhibiting effect on Candida albicans and the inhibition was in a dose-dependent manner.
出处
《北京口腔医学》
CAS
2011年第2期70-73,共4页
Beijing Journal of Stomatology
关键词
抗菌肽
白色念珠菌
义齿性口炎
抗真菌活性
Antimicrobial peptides
Synthetic
Candida albicans
Denture stomatitis
Antifungal activity
