摘要
利用cDNA-AFLP分析籼稻明恢86应答稻纵卷叶螟取食基因差异表达,发现1个与植物同源结构域(PHD)锌指蛋白高度同源的TDF,分离获得该TDF对应的粳稻全长cDNA.该全长cDNA与籼稻、玉米、蓖麻、葡萄、拟南芥和大豆等作物PHD锌指蛋白推定氨基酸序列的同源性分别为98.43%、86.01%、54.58%、57.02%、57.51%和55.88%.荧光定量PCR研究表明,日本晴(粳稻)叶片中该基因的表达也受稻纵卷叶螟取食的诱导,推测该基因与籼稻和粳稻应答稻纵卷叶螟取食密切相关.
eDNA AFLP was applied to display the differentially expressed genes in rice Indica Minghui 63 against Cnaphalocrocis medinalis, then a TDF homologous to PHD zinc finger protein was found, its corresponding full length cDNA in Japonica was ampli fied by RT PCR, the corresponding amino acids sequence shares 98.43%, 86.01%, 54.58%, 57.02%, 57.51% and 55.88% ho mology to Indica rice, Zea mays, Ricirtus communis, Vitis vinifera, Arabidopsis thaliana and Glycine max repectively. The result of real time RT-PCR showed that the PHD finger protein was also induced by C. medinalis, suggesting the involvement in defence reac tion in both Indica and Japonica rice.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2011年第2期161-164,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金面上项目(2007J0046)
关键词
稻纵卷叶螟
PHD锌指蛋白
表达分析
Cnaphalorocis medinali
PHD finger protein
expression analysis
