摘要
目的:探讨电针治疗阿尔茨海默病的可能作用机制。方法:将13月龄的淀粉样前体蛋白(APP)转基因小鼠随机分为模型组和电针治疗组,以相同月龄和背景的C 57 BL/6小鼠作对照组。电针治疗组取"百会""涌泉"穴,每次留针15 min,隔日1次,针刺3个月。以LashleyⅢ水迷宫测定各组小鼠的学习记忆能力,以免疫组化方法检测海马β淀粉样蛋白(amyloidβprotein,Aβ)、低密度脂蛋白相关受体1(low densi-ty lipoprotein receptor-related protein 1,LRP 1)的表达。结果:模型组水迷宫游出时间较对照组延长(P<0.05),海马沟微血管壁Aβ1-42表达的累积吸光度较对照组升高(P<0.01),海马沟微血管壁LRP 1表达较对照组减低(P<0.01);而电针治疗组的水迷宫游出时间明显低于模型组,海马沟微血管壁Aβ1-42表达低于模型组,海马沟微血管壁LRP 1表达高于模型组(均P<0.05)。结论:电针治疗可能通过提高脑微血管壁Aβ受体LRP 1的清除转运能力,降低APP转基因鼠脑微血管壁的Aβ沉积,从而改善其学习、记忆能力。
Objective To investigate the effect of electroacupuncture （EA） of ＂Baihui＂ （GV20） and ＂Yongquan＂ （KI 1） on the expression of amyloid β protein （AIS） and low density lipoprotein （LDL） receptor-related protein 1 （LRP 1 ） in the hippocampal sulcus microvessels in amyloid precursor protein （APP） 695 V 717 I transgenic mice, so as to study its mechanism under-lying relief of Alzheimer Disease （AD）. Methods Twelve APP 695 V 717 I transgenic mice were randomly divided into model group and EA group. Six C 57 BL 6 mice were used as the control group. EA （2Hz/100Hz, 3-5mA） was applied to＂Baihui＂ （GV 20） and ＂Yongquan＂ （KI 1 ） for 15 min, once every other day for three months. The learning-memory ability of mice was detected by using Lashley Ⅲ water maze system. The expression level of Aβ1-42, and LRP 1 in the hippocampal sulcus microvessels were measured by immunohistochemistry. Results Water maze test showed that the swimming duration from the start to the goal box （terminal） in the Lashley Ⅲ water maze was significantly longer in the model group than in the control group （P〈0.05）, suggesting a markedly lower learning-memory capacity of APP 695 V 317 I transgenic mice. Compared with the model group, the swimming duration in the EA group was decreased considerably （P〈0.05）. The integrated optical density （IOD） value of hippocampal Aβ1-42 immunoreaction （IR） positive products in the model group was significantly higher than that in the control group （ P〈 0. 01） and the IOD value of hippocampal LRP 1 IR-positive products in the modet group was apparently Io-wer than that in the control group （ P〈0.01 ）. In comparison with the model group, the IOD value of Aβ1-42 IR-positive products in the EA group was obviously lower than that in the model group （P〈0.05）, while that of LRP 1 IR-positive products in the EA group was significantly higher than that in the model group （ P〈0.05）, suggesting down-regulation of hippocampal Aβ1-42 expression and up-regulation of LRP 1 expression after EA. and reduction of deposition of Aβ in the cerebral microvessels after EA. Conclusion EA can improve the learning-memory capacity of APP transgenic mice, which is closely related to its effects in up-regulating hippocampal LRP 1 expression and down-regulating hippocarnpal Aβ-42 expression.
出处
《针刺研究》
CAS
CSCD
北大核心
2011年第2期95-100,共6页
Acupuncture Research
基金
高等学校博士学科点专项科研基金资助项目(No.20060026015)
