摘要
目的:探讨核因子κB(NF-κB)与喉癌循环肿瘤细胞(CTC)发生之间的相关性,并观察NFκ-B抑制剂PDTC对CTC的影响及可能机制。方法:裸鼠皮下接种喉癌Hep-2细胞,建立喉癌裸鼠模型。将60只裸鼠随机分为实验组(PDTC预处理组)、对照组(生理盐水组)及空白组(不接种喉癌细胞),每组各20只。肿瘤接种成功8周后处死裸鼠,采用RT-PCR法,以CK19mRNA为标记物检测各组中CTC的表达;取出肿瘤组织,测定其体积,免疫组织化学方法观察喉癌组织NF-κB的表达及激活情况,实时荧光定量PCR检测各组喉癌组织中血管内皮生长因子-C和基质金属蛋白酶-9 mRNA的表达。结果:CTC发生与NF-κB的表达率无明显关联,在CTC表达阳性的喉癌组织中,NFκ-B阳性率为90%,而在CTC表达阴性的喉癌组织中,NF-κB阳性率为56.7%(P>0.05),但其与NF-κB的活性有关;PDTC可抑制NF-κB的激活,减少喉癌裸鼠模型中CTC的发生率,PDTC组CTC阳性率为5%,明显低于对照组CTC的阳性率45%(P<0.01)。结论:PDTC可减少喉癌裸鼠模型中CTC的发生率,其作用可能得益于PDTC降低了喉癌发生转移的能力,其机制可能为PDTC通过抑制NF-κB活性而使血管内皮生长因子-C和基质金属蛋白酶-9表达下调,减少肿瘤组织新生血管形成及降低喉癌侵袭力有关。
Objective：To investigate the correlation of the nuclear factor（NF-κB） and laryngocarcinoma circulating tumor cells（CTC）,observe nuclear factor inhibitor PDTC on laryngeal cancer CTC and its possible mechanism.Method：In order to establish of laryngocarcinoma nude mice model,The nude mice inoculated with laryngocarcinoma Hep-2 cells.To 50 nude mice were randomly divided into experimental group（PDTC pretreatment group）,the control group（saline group）,and Sham group（not inoculated carcinoma cells）,tumor inoculated mice were labeled with CK-19 CTC markers,and sacrificed after 8 weeks,then RT-PCR detect CK-19mRNA expression in peripheral blood as to understand the expression of laryngocarcinoma CTC;removed tumor to determine its size,immunohistochemical expression of NF-κB in laryngocarcinoma,real-time fluorescence Quantitative PCR detection of laryngocarcinoma VEGF and MMP-9mRNA expression.Result：CTC occurrence and the expression of NF-κB was not obviously associated,expression in the CTC-positive laryngeal carcinoma,NF-κB-positive rate was 90%,while the expression of negative CTC laryngocarcinoma,NF-κB-positive rate was 56.7%（P 0.05）,but connected with the activity of NF-κB;PDTC can inhibit NF-κB activation,reduce the incidence of CTC in laryngocarcinoma nude mice model,PDTC group CTC positive rate of 5%,significantly lower than the control group CTC positive rate of 45%（P0.01）.Conclusion：PDTC can reduce the incidence of CTC in the laryngocarcinoma nude mouse model,its role may benefit from PDTC reduced the ability of laryngocarcinoma metastasis,which may be as PDTC inhibit NF-κB activity,thus make VEGF-C and the expression of MMP-9 down,reducing angiogenesis and reduce tumor invasion of the larynx.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2011年第9期419-422,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
喉肿瘤
循环肿瘤细胞
核因子-ΚB
核因子抑制剂
laryngocarcinoma
circulating tumor cells
nuclear factor-κB
nuclear factor inhibitor
