摘要
为了明确番茄叶片光合作用及蔗糖代谢对夜间亚低温的响应机制,系统研究了9℃夜间亚低温及其恢复对番茄净光合速率、各器官干物质量、碳水化合物积累、蔗糖代谢酶活性的影响。结果表明：9℃夜间亚低温降低了番茄净光合速率和各器官的干物质量,增加了淀粉、果糖、葡萄糖、蔗糖和总糖的含量;抑制了番茄中酸性转化酶（AI）、中性转化酶（NI）、蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性;夜间9℃亚低温处理6d内,在15℃适温恢复9d时番茄光合速率、各器官干物质量、淀粉、果糖、葡萄糖含量等均可恢复到对照水平,但处理9d时再恢复9d,光合速率、淀粉和果糖含量、SS活性均未能恢复到对照水平,特别是处理6~9d时再恢复9d,蔗糖、蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）活性均未恢复到对照水平,且亚低温处理时间越长恢复所需时间越长。从蔗糖和淀粉含量分别与净光合速率和SPS活性呈极显著负相关关系的结果看,夜间亚低温导致的净光合速率的降低可用＂光合末端产物的反馈抑制＂来解释,说明源叶中光合末端产物的积累可能是光合效率降低的直接原因之一。
In a bid to discuss the response mechanism of carbohydrate metabolism in tomato leaves to short-term sub-low night temperature.Dynamic changes of accumulation of each organ dry matter,carbohydrate contents and sucrose metabolizing enzyme activities in tomato（Lycopersicon esculentum Mill.）leaves under short-term sub-low night temperature at 9 ℃ for 3 to 9 days and then recover for 9 days at 15 ℃ were studied.The results showed that short-term sub-low night temperature at 9 ℃ decreased the dry matter content of the each organ,increased fructose content,glucose content,sucrose content and total sugar as well as starch content,inhibited the activities of acid invertase（AI）,neutral invertase（NI）,sucrose synthase（SS）and sucrose phosphate synthase（SPS）.The net photosynthetic rate,dry matter content of the each organ,starch,fructose,glucose content,and so on were all reversible at 15 ℃ for 9 days after treating 6 days at 9 ℃,but the net photosynthetic rate,starch,fructose content and sucrose synthase（SS）were irreversible for recovery 9 days after treating 9 days.Especially,sucrose content,sucrose synthase（SS）and sucrose phosphate synthase（SPS）could not come back to control for recovery 9 days after treating 6-9 days,and the slower recovery rate was,the longer treatment time was.There were significnatly negative linear correlations between sucrose and starch content and Pn,as well as sucrose content and SPS activity.Reduced photosynthesis under sub-low night temperature could be explained by feedback inhibition of end-productandit and it was suggested that the accumulation of end-product in leaves could be the direct factor in regulating photosynthesis.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第4期683-691,共9页
Acta Horticulturae Sinica
基金
大宗蔬菜产业技术体系岗位科学家专项(Nycytx-35-gw23)
辽宁省重大科技攻关项目(2010215003)
辽宁省教育厅优秀人才基金项目(2007R40)
关键词
番茄
夜间亚低温
恢复
蔗糖代谢
tomato
short-term sub-low temperature
recovery
sucrose-metabolizing
