期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

银屑病皮损及正常人皮肤中Akt1,Akt2和Akt3的表达 被引量：9

The Expression of Akt1,Akt2,Akt3 in the Lesions of Psoriasis Vulgaris
原文传递
导出
摘要 目的探讨Akt三种亚型Akt1,Akt2和Akt3的表达在寻常性银屑病发病中的意义。方法采用免疫组化法检测30例寻常性银屑病皮损及15例正常皮肤中Akt1,Akt2和Akt3的表达,并利用计算机图像采集与分析系统对免疫组化结果进行平均光密度测定。结果免疫组化结果显示,与正常人表皮相比,寻常性银屑病皮损中Akt1(0.2170±0.0144)和Akt2(0.2157±0.0150)的表达差异无统计学意义(P>0.05),而Akt3(0.2990±0.0165)的表达却明显高于正常皮肤,差异有统计学意义(P<0.01)。结论寻常性银屑病皮损内Akt活性的升高可能与Akt3的表达增强有关。 Objective To investigate the significance of the expression of three isoforms of Akt in the pathogenesis of psoriasis. Methods ＇ Immunohistochemistry method was used to detect the expression of Akt1, Akt2, Akt3 in lesions from 30 patients with psoriasis vulgaris and skin from 15 normal people. The expression levels of Akt1, Akt2, Akt3 were analyzed by measurement of the average optical density of immunostaining. Results The expression of both Aktl （0. 2170 ±0.0144）. and Akt2（0. 2157 ±0.0150） in psoriatic lesions had no obvious differences compared with that of normal skin （P 〉 0.05, respectively）. However, the expression of Akt3 （0.2990 ± 0. 0165） in psoriatic lesions was significantly higher than that in normal skin （P 〈 0.01 ）. Conclusion The increased expression of Akt3 might be correlated to the elevation of phosphorylated Akt and Akt activity in psoriatic lesions.
作者 蔓小红 张晓艳 李红艳 徐波 唐娟 陈扬鑫 潘琳
机构地区 卫生部中日友好医院皮肤科 卫生部中日友好医院临床研究所
出处 《中国皮肤性病学杂志》 CAS 北大核心 2011年第5期338-340,共3页 The Chinese Journal of Dermatovenereology
基金 国家自然科学基金资助项目(30872266) 北京自然科学基金资助项目(7092094)
关键词 AKT1 AKT2 Akt3 银屑病 Akt1 Akt2 Akt3 Psoriasis
分类号 R758.63 [医药卫生—皮肤病学与性病学]
  • 相关文献

参考文献11

  • 1Song G, Ouyang G, Bao S. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med, 2005, 9( 1 ) :59 -71.
  • 2张晓艳,周平,尤立平,于长安,潘琳,马圣清.银屑病皮损表皮内Akt的激酶活性增强[J].中华皮肤科杂志,2009,42(6):413-416. 被引量：9
  • 3Le Page C. Koumakpayi 1H, Alam-Fahmy M, et al. Expression and localisation of Akt-1, Akt-2 and Akt-3 eorrelate with clinical outcome of prostate cancer patients[J]. Br J Cancer, 2006, 94(12) :1906 - 1912.
  • 4Gonzalez E, McGraw TE. The Akt kinases: isoforn specificity in metabolism and cancer[ J ]. Cell Cycle, 2009, 8 (16) :2502 - 2508.
  • 5Chen WS, Xu PZ, Gottlob K, et al. Growth retardation and increased apoptosis in mice with homozygous disruption of the Aktl gene [ J ]. Genes Dev, 2001, 15(17) :2203 -2208.
  • 6Garofalo RS, Orena SJ, Rafidi K, et al. Severe diabetes, age-dependent loss of adipose tissue, and mild growth deficiency in mice lacking Akt2/PKBbeta [ J ]. J Clin Invest, 2003, 112 ( 2 ) : 197 - 208.
  • 7Stambolic V,Woodgett JR. Functional distinctions of p rolein kinase B /Akt isoforms defined bv their influenece on eell migration [ J ]. Trends Cell Biol, 2006, 16 (9) : 461 -466.
  • 8Tschopp O, Yang ZZ, Brodbeck D, et al. Essential role of protein kinase Bgamma ( PKBgamma/Akt3 ) in postnatal brain development but not in glucose homeostasis[ J ]. Development, 2005, 132 ( 13 ) :2943 - 2954.
  • 9Sale EM,Hodgkinson CP,Jones NP,et al. A new strategy for studying protein kinase B and its three isohmns. Role of protein kinase B in phosphorylating glycogen synthase kinase-3, tuberin, WNKI, aod ATP citrate lyase[ J]. Bioehemistry, 2006, 45( 1 ) :213 -223.
  • 10张晓艳,马圣清,柳惠图.银屑病患者皮损中磷脂酰肌醇3激酶过表达[J].中华皮肤科杂志,2002,35(2):97-98. 被引量：4

二级参考文献22

  • 1周颖,王建,贺福初.蛋白激酶B(PKB/Akt)的结构、调控与功能[J].生命的化学,2006,26(3):226-228. 被引量：13
  • 2Wymann MP, Zvelebil M, Laffargue M. Phosphoinositide 3- kinase signaling--which way to target? Trends Pharmacol Sci, 2003, 24(7): 366-376.
  • 3Cantley LC. The phosphoinositide 3-kinase pathway. Science, 2002, 296(5573): 1655-1657.
  • 4Fabre S, Lang V, Harriague J, et al. Stable activation of phospha- tidylinositol 3-kinase in the T cell immtmological synapse stimulates Akt signaling to FoxO1 nuclear exclusion and cell growth control. J Immunol, 2005, 174(7): 4161-4171.
  • 5Philp AJ, Campbell IG, Leet C, et al. The phosphatidylinositol 3'- kinase p85 alpha gene is an oncogene in human ovarian and colon tumors. Cancer Res, 2001, 61(20): 7426-7429.
  • 6Terragni J, Graham JR, Adams KW, et al. Phosphatidylinositol 3-kinase signaling in proliferating cel!s maintains an antiapoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NF kappaB transcription factors. BMC Cell Biol, 2008, 9: 6.
  • 7Cantley LC. The phosphoinositide 3-kinase pathway. Science, 2002, 296(5573): 1655-1657.
  • 8Pareellier A, Tintignac LA, Zhuravleva E, et al. PKB and the mitochondria: AKTing on apoptosis. Cell Signal, 2008, 20 (1): 21-30.
  • 9Shtilbans V, Wu M, Burstein DE. Current overview of the role of Akt in cancer studies via applied immunohistochemistry. Ann Diagn Pathol, 2008, 12(2): 153-160.
  • 10Xu S, Cwyfan-Hughes SC, van der Stappen JW, et al. Altered insulin-like growth factor-II (IGF-II) level and IGF-binding protein-3 (IGFBP-3) protease activity in interstitial fluid taken from the skin lesion of psoriasis. J Invest Dermatol, 1996, 106 (1): 109-112.

共引文献11

同被引文献124

引证文献9

二级引证文献33

中国皮肤性病学杂志
2011年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部