摘要
目的研究饥饿诱导的自噬对巨噬细胞吞噬鸡红细胞的影响,初步探讨自噬在巨噬细胞吞噬功能中的作用。方法实时动态观察巨噬细胞吞噬鸡红细胞的过程,单丹磺酰尸胺(MDC)荧光染色标记巨噬细胞的自噬体,激光扫描共聚焦显微镜下进行观察和定量分析。Western blot方法检测巨噬细胞的微管相关蛋白1轻链3(LC3)的表达,HE染色观察自噬对其吞噬功能的影响。结果巨噬细胞活跃地吞噬鸡红细胞,吞噬后6 h MDC荧光染色阳性率明显高于对照组。饥饿诱导巨噬细胞LC3Ⅱ表达增高,并可以被3-甲基腺嘌呤(3-MA)抑制,饥饿3 h后的巨噬细胞对鸡红细胞的吞噬率与对照组相比无明显变化。自噬被3-MA抑制后,巨噬细胞对鸡红细胞的吞噬率明显低于对照组。结论巨噬细胞活跃地吞噬鸡红细胞,吞噬后自噬明显增强;自噬对巨噬细胞吞噬功能有重要作用。
Objective To investigate the effect of autophagy induced by starvation on phagocytosis of macrophages and explore the effect of autophagy on phagocytosis of macrophages.Methods The dynamic process of macrophage phagocytosis of chicken red blood cells was observed by living cells station.The autophagosomes of macrophages were labeled with monodansylcaolaverine（MDC） staining and quantitated using laser scanning confocal microscopy.The microtubule-associated protein 1 light chain 3（LC3） expression of macrophages was measuerd by Western blot and the effects of autophagy on phagocytosis of macrophages were analyzed by HE staining.Results The percentage of autophagosome-positive of macrophages after phagocytosis of chicken red blood cells 6 h was significant increased compared with control groups.The LC3Ⅱ expression of macrophages induced by starvation was significant increased than that inhibited by 3-methyladenine（3-MA）.The phagocytic rates of macrophages which were starvated 3 h were no changes compared with control groups,but when starvated and blocked by 3-MA were significantly lower than that in control groups.Conclusion Autophagy of macrophages is significantly enhanced after phagocytosis of chicken red blood cells.Autophagy plays important roles in phagocytosis of macrophages.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第5期401-404,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30670936)
关键词
巨噬细胞
自噬
吞噬作用
macrophage
autophagy
phagocytosis
