摘要
目的 探讨表没食子儿茶素没食子酸酯（EGCG）对人胰腺癌细胞株SW1990增殖及细胞凋亡、细胞周期的影响.方法 采用四甲基偶氮唑蓝（MTT）比色法检测不同浓度EGCG（6.25、12.5、25、50、100μg/ml）对体外培养的SW1990细胞增殖的影响;采用流式细胞仪检测EGCG（25μg/ml）对SW1990细胞凋亡及不同浓度的EGCG（0、10、20、30、40、50 μg/ml）对SW1990细胞周期的影响.结果 不同浓度EGCG（0、25、50μg/ml）作用SW1990细胞24 h后,吸光度值（A492）分别为0.46±0.04、0.42±0.04、0.27±0.03,48 h后分别为0.48±0.02、0.31±0.03、0.16±0.02,72 h后分别为0.51±0.01、0.24±0.04、0.14±0.04,EGCG呈浓度及时间依赖性抑制SW1990的增殖（P＜0.01）.25 μg/ml EGCG作用于SW1990细胞24、48、72 h后的细胞凋亡率分别为（8.33±1.15）%、（19.77±0.81）%、（29.17±0.75）%,而对照组相应的细胞凋亡率分别为（2.77±0.45）%、（3.20±0.26）%、（3.67±0.35）%,两组差异具有统计学意义（P＜0.01）.0、20、50 μg/ml EGCG作用SW1990细胞24 h后,G0/G1期细胞分别占（57.59±0.97）%、（62.99±1.91）%、（68.87±1.88）%,随着EGCG浓度的增加,Go/G1期细胞比例明显增加,而S期和G2/M期细胞比例相应下降（P＜0.01）.结论 EGCG能明显抑制SW1990细胞的增殖,其机制可能与其诱导SW1990细胞凋亡及调控细胞周期有关.
Objective To investigate the apoptosis-inducing effect and anti-proliferative effect of epigallocatechin-3-gallate （EGCG） on human pancreatic cancer cell SW1990 in vitro. Methods The effect of proliferation was evaluated by MTT after the SW1990 cells in vitro were incubated with different concentrations of EGCG （6.25, 12.5, 25, 50, 100 μg/ml）. The apoptosis-inducing effect was determined by flow cytometry after the cells were treated with 25 μg/ml of EGCG. The cell cycle of SW1990 cells was detected by flow cytometry after the cells incubated with different concentrations of EGCG （0, 10, 20, 30, 40, 50 μg/ml）.Results After SW1990 cell were treated with different concentrations of EGCG （0, 25, 50 μg/ml）, the values of A492 were 0.46 ±0.04,0.42 ±0.04,0.27 ±0.03 at 24 h; 0.48 ±0.02, 0.31 ±0.03,0.16 ±0.02at 48 h; 0.51 ±0.01,0.24 ±0.04,0. 14 ±0.04 at 72 h. EGCG inhibited the proliferation of SW1990 in a doseand time-dependant manner（P ＜0.01 ）. The apoptotic rates at 24, 48, 72 h were （8.33 ± 1.15 ）%, （19.77 ±0.81 ）%, （29.17 ± 0.75 ）% in the EGCG treatment group; while the corresponding values were （2.77 ±0.45 ） %, （3.20 ± 0.26 ） %, （3.67 ± 0.35 ） % in the control group; and the difference was statistically significant （P ＜0.01 ）. After 0, 20, 50 μg/ml of EGCG treatment for 24 h, the percentages of SW1990 cellsin G0/G1 stage were （57.59 ±0.97）%, （62.99 ± 1.91 ）%, （68.87 ± 1.88）%, and the percentages of SW1990 cells in G0/G1 stage increased with the increase of concentrations of EGCG, while the percentages of SW1990 cells in G2/M stage decreased with the increase of concentrations of EGCG （P ＜0.01 ）. Conclusions EGCG can significantly inhibit the proliferation of SW1990 cells. The mechanism may be related to the apoptosis-inducing effect and the regulation of the cell cycle of the SW1990 cells.
出处
《中华胰腺病杂志》
CAS
2011年第2期101-103,共3页
Chinese Journal of Pancreatology
