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辐射诱导性miR-34a的表达对细胞辐射敏感性的影响 被引量：6

Effect of irradiation-induced miR-34a expression on irradiation-sensitivity of cells
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摘要 目的探讨miR-34a的表达与细胞、组织辐射敏感性的关系。方法采用RT-PCR检测辐射前后不同组织、细胞中miR-34a的表达量;利用流式细胞术检测细胞凋亡率,MTT法检测细胞活力;在肿瘤细胞U87MG中转染miR-34amimics上调miR-34a的表达并检测其细胞活力;在正常细胞HEK293中转染miR-34a inhibitors下调miR-34a的表达水平并检测其细胞凋亡率。结果照前辐射敏感性高的细胞miR-34a表达水平高,辐射敏感性低的细胞miR-34a表达水平低;照后辐射敏感性高的细胞miR-34a表达上调幅度远高于辐射敏感性低的细胞。上调miR-34a表达水平使得肿瘤细胞U87MG照后细胞活力降低,下调miR-34a表达使得正常细胞HEK293照后细胞凋亡率降低。结论细胞辐射敏感性与miR-34a的表达水平正相关,辐射敏感性高的细胞照后miR-34a的表达上调幅度更大;上调miR-34a表达水平对肿瘤细胞U87MG有明显的辐射增敏作用,下调miR-34a表达对正常细胞HEK293有明显的辐射防护作用。 Objective To investigate the relationship between miR-34a expression and the irradiation-sensitivity of cells and tissues.Methods RT-PCR was used to detect the miR-34a expression in different cells and tissues before and after irradiation;flow cytometry was employed to examine the apoptosis of cells and MTT assay was used to observe the cell viability.The U87MG tumor cells were transfected with miR-34a mimics to promote miR-34a expression and the cell viability was observed 24 h later.The HEK293 renal epithelial cells were transfected with miR-34a inhibitors to decrease miR-34a expression and the cells apoptosis was observed 24 h later.Results The expression of miR-34a was higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells before irradiation;the increase of miR-34a expression was greatly higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells 24 h after irradiation.The up-regulation of miR-34a decreased the viability of U87MG tumor cells,and down-regulation of miR-34a decreased the apoptosis of HEK293 cells.Conclusion The irradiation-sensitivity of cells is positively associated with miR-34a;cells with high irradiation-sensitivity have a greater up-regulation of miR-34a expression after irradiation.Increased miR-34a expression can noticeably increase the irradiation-sensitivity of U87MG tumor cells and decreased miR-34a expression has a irradiation prevention effect on normal HEK293 cells.
作者 程赢 和生辉 刘聪 周传丰 崔建国 孙顶 蔡建明 李百龙 高福
机构地区 第二军医大学海军医学系放射医学教研室
出处 《第二军医大学学报》 CAS CSCD 北大核心 2011年第4期368-371,共4页 Academic Journal of Second Military Medical University
基金 国家自然科学基金(30970679 31070761)~~
关键词 MIR-34A 辐射耐受性 辐射增敏药 辐射防护 miR-34a radiation tolerance radiation-sensitizing agents radiation protection
分类号 R818 [医药卫生—放射医学]
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参考文献11

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二级参考文献39

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共引文献15

同被引文献63

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引证文献6

二级引证文献6

第二军医大学学报
2011年 第4期

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