摘要
目的研究蜂胶水提物对氧化型低密度脂蛋白诱导的平滑肌细胞凋亡的影响。方法培养人脐动脉平滑肌细胞,培养的细胞随机分为五组:对照组、模型组和三种浓度的蜂胶水提物干预组。采用高效液相色谱法测定细胞内总胆固醇和游离胆固醇含量,从中得出胆固醇酯含量,根据细胞蛋白定量。采用流式细胞仪Annexin V/PI双染法检测细胞凋亡率。结果模型组细胞内胆固醇酯含量高于对照组(P<0.01),50 mg/L、100 mg/L及200mg/L蜂胶水提物干预组细胞内胆固醇酯含量均低于模型组(P<0.01),且随蜂胶水提物浓度的增高细胞内胆固醇酯含量有减少的趋势;模型组细胞凋亡率高于对照组(P<0.01),50 mg/L、100 mg/L及200 mg/L蜂胶水提物干预组细胞凋亡率均低于模型组(P<0.01),且随蜂胶水提物浓度的增高细胞凋亡率有降低的趋势。细胞内胆固醇酯含量与细胞凋亡率之间存在正相关(r=0.964,P<0.01)。结论蜂胶水提物能够降低氧化型低密度脂蛋白诱导的人脐动脉平滑肌细胞凋亡,可能与蜂胶水提物抑制细胞内胆固醇酯聚集有关。
Aim To investigate the effect of water extract propolis（WEP） on apoptosis in human umbilical arterial smooth muscle cell（HUASMC）,and explore the mechanism of water extract propolis on inhibition of atherosclerosis and it＇s implications. Methods HUASMC were cultured and identified by immunocytochemistry technique.Cultured HUASMC were randomly divided into 5 groups： control group,model group and WEP groups（treated with 50 mg/L,100 mg/L and 200 mg/L WEP respectively）.Intracellular total cholesterol（TC） and free cholesterol（FC） were measured by high performance liquid chromatogram,the content of cholesterol ester（CE） was obtained by subtracting the FC from TC.Cellular apoptosis index was tested by flow cytometry. Results The content of intracelluar CE in model group was more than that in control group（P0.01）.The content of intracellular CE in 50 mg/L,100 mg/L and 200 mg/L WEP groups were less than that in the model group（P0.01）,and with the increase of the concentration of WEP the content of intracellular CE showed the decreasing tendency.The cell apoptosis index in model group was higher than that in control group（P0.01）.The cell apoptosis index in 50 mg/L,100 mg/L and 200 mg/L WEP groups were lower than that in the model group（P0.01）,and with the increase of the concentration of WEP the cell apoptosis index showed the decreasing tendency.The content of intracellular CE was correlated with the cell apoptosis（r=0.964,P0.01）. Conclusion WEP can reduce the apoptosis of HUASMC induced by ox-LDL which may be concerned with the inhibination of intracellular CE accumulation.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2011年第3期202-205,共4页
Chinese Journal of Arteriosclerosis
基金
泰山医学院课题(2001086)
