摘要
目的研究P-选择素基因单核苷酸多态性与急性心肌梗死易感性之间的关系;同时分析P-选择素基因型及血清水平与急性心肌梗死的相关性。方法以150例急性心肌梗死患者和160例健康对照者为研究对象,应用聚合酶链反应限制片长多态性和DNA测序法对P-选择素基因-2123 G/C单核苷酸多态性进行基因分型,同时采用酶联免疫吸附法检测血清P-选择素水平。结果急性心肌梗死组血清P-选择素水平明显高于对照组(P<0.05),P-选择素基因-2123 G/C多态性在急性心肌梗死组和对照组中的分布差异存在显著性(P<0.05),对等位基因频率的相对风险分析发现,-2123 C等位基因携带者患急性心肌梗死的风险是G等位基因携带者的1.625倍(OR=1.625,95%CI为1.177～2.244,P=0.003);携带-2123 C等位基因的急性心肌梗死患者血清P-选择素水平显著高于不携带者(P<0.05)。结论 P-选择素基因-2123 G/C多态性与急性心肌梗死的发病可能具有相关性,其中-2123 C等位基因可能是急性心肌梗死的遗传易感基因,携带-2123 C等位基因的个体可能通过促进P-选择素的高度表达进而增加急性心肌梗死的发病风险。
Aim To investigate the association of P-selectin gene single nucleotide polymorphisms（SNP） and susceptibility to acute myocardial infarction（AMI） in a Chinese population,and to analyze association of serum levels and genotypes of P-selectin with AMI. Methods P-selectin gene-2123 G/C single nucleotide polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and DNA sequencing among 150 AMI patients and 160 age and sex matched controls in a Chinese population,and serum level of P-selectin was determined by enzyme-linked immunosorbent assay（ELISA）. Results Serum levels of P-selectin in AMI patients were significantly higher than those of controls（P0.05）,the distributions of P-selectin gene-2123 G/C polymorphism were significantly different between AMI group and control group（P0.05）. The relative risk suffered from AMI of-2123 C allele was 1.625 times of the G allele carriers（OR=1.625,95%CI was 1.177～2.244,P=0.003）;the serum levels of P-selectin C allele carriers was significantly higher than no carriers（P0.05）. Conclusions P-selectin gene-2123 G/C polymorphism was associated with AMI,-2123 C allele is an important genetic susceptibility gene for AMI. In which the P-selectin C allele carriers may increase risk by enhancing the P-selectin expression in the pathogenesis of AMI.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2011年第2期151-154,共4页
Chinese Journal of Arteriosclerosis
关键词
P-选择素
急性心肌梗死
单核苷酸多态性
基因型频率
P-Selectin
Acute Myocardial Infarction
Single Nucleotide Polymorphism
Genotype Frequency
