摘要
AIM:To investigate the effects of the Chinese herbal decoction,Yi-Qi-Zeng-Min-Tang(YQZMT),on insulin resistance in type 2 diabetic rats.METHODS:Sprague-Dawley rats were divided into two dietary regiments by feeding either normal pellet diet(NPD) or high fat diet(HFD).Four weeks later,the HFD-fed rats were injected intraperitoneally with lowdose streptozotocin(STZ).Rats with non-fasting blood glucose level ≥ 16.67 mmol/L were considered type 2 diabetic and further divided into five subgroups:the type 2 diabetes model group,low-dose,medium-doseand high-dose YQZMT groups,and rosiglitazone group.Age-matched NPD-fed rats served as controls.YQZMT or rosiglitazone were administered for 8 wk.Intraperitoneal glucose and insulin tolerance tests were performed before and after the treatment to measure the glucose tolerance and insulin sensitivity.Serum levels of biochemical parameters,adipocytokines,such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),as well as free fatty acids(FFAs),were also analyzed.RESULTS:There was significant elevation of insulin resistance and serum levels of fasting glucose(12.82 ± 1.08 mmol/L vs 3.60 ± 0.31 mmol/L,P < 0.01),insulin(7197.36 ± 253.89 pg/mL vs 4820.49 ± 326.89 pg/mL,P < 0.01),total cholesterol(TC)(8.40 ± 0.49 mmol/L vs 2.14 ± 0.06 mmol/L,P < 0.01),triglyceride(2.24 ± 0.12 mmol/L vs 0.78 ± 0.05 mmol/L,P < 0.01),low-density lipoprotein cholesterol(LDL-c)(7.84 ± 0.51 mmol/L vs 0.72 ± 0.04 mmol/L,P < 0.01) and decrease in high-density lipoprotein cholesterol(HDL-c)(0.57 ± 0.03 mmol/L vs 1.27 ± 0.03 mmol/L,P < 0.01) in the low-dose STZ and high-fat diet induced type 2 diabetic group when compared with the control group.Administration of YQZMT induced dose-and timedependent changes in insulin resistance,glucose and lipid profile,and reduced levels of FFA,TNF-α and IL-6 in the type 2 diabetic rats.After the treatment,compared with the diabetic group,the insulin resistance was ameliorated in the high-dose YQZMT(2.82 g/100 g per day) group,with a significant reduction in serum glucose(12.16 ± 1.00 mmol/L vs 17.65 ± 2.22 mmol/L,P < 0.01),homeostasis model assessment of basal insulin resistance(22.68 ± 2.37 vs 38.79 ± 9.02,P < 0.05),triglyceride(0.87 ± 0.15 mmol/L vs 1.99 ± 0.26 mmol/L,P < 0.01),TC(3.31 ± 0.52 mmol/L vs 6.50 ± 1.04 mmol/L,P < 0.01) and LDL-c(2.47 ± 0.50 mmol/L vs 6.00 ± 1.07 mmol/L,P < 0.01),and a signif icant increase in HDL-c(0.84 ± 0.08 mmol/L vs 0.50 ± 0.03 mmol/L,P < 0.01).But the body weight was not changed signif icantly.CONCLUSION:YQZMT,which ameliorates insulin resistance and does not cause increase in body weight,may be a suitable therapeutic adjunct for the treatment of type 2 diabetes.
AIM：To investigate the effects of the Chinese herbal decoction,Yi-Qi-Zeng-Min-Tang（YQZMT）,on insulin resistance in type 2 diabetic rats.METHODS：Sprague-Dawley rats were divided into two dietary regiments by feeding either normal pellet diet（NPD） or high fat diet（HFD）.Four weeks later,the HFD-fed rats were injected intraperitoneally with lowdose streptozotocin（STZ）.Rats with non-fasting blood glucose level ≥ 16.67 mmol/L were considered type 2 diabetic and further divided into five subgroups：the type 2 diabetes model group,low-dose,medium-doseand high-dose YQZMT groups,and rosiglitazone group.Age-matched NPD-fed rats served as controls.YQZMT or rosiglitazone were administered for 8 wk.Intraperitoneal glucose and insulin tolerance tests were performed before and after the treatment to measure the glucose tolerance and insulin sensitivity.Serum levels of biochemical parameters,adipocytokines,such as tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,as well as free fatty acids（FFAs）,were also analyzed.RESULTS：There was significant elevation of insulin resistance and serum levels of fasting glucose（12.82 ± 1.08 mmol/L vs 3.60 ± 0.31 mmol/L,P 〈 0.01）,insulin（7197.36 ± 253.89 pg/mL vs 4820.49 ± 326.89 pg/mL,P 〈 0.01）,total cholesterol（TC）（8.40 ± 0.49 mmol/L vs 2.14 ± 0.06 mmol/L,P 〈 0.01）,triglyceride（2.24 ± 0.12 mmol/L vs 0.78 ± 0.05 mmol/L,P 〈 0.01）,low-density lipoprotein cholesterol（LDL-c）（7.84 ± 0.51 mmol/L vs 0.72 ± 0.04 mmol/L,P 〈 0.01） and decrease in high-density lipoprotein cholesterol（HDL-c）（0.57 ± 0.03 mmol/L vs 1.27 ± 0.03 mmol/L,P 〈 0.01） in the low-dose STZ and high-fat diet induced type 2 diabetic group when compared with the control group.Administration of YQZMT induced dose-and timedependent changes in insulin resistance,glucose and lipid profile,and reduced levels of FFA,TNF-α and IL-6 in the type 2 diabetic rats.After the treatment,compared with the diabetic group,the insulin resistance was ameliorated in the high-dose YQZMT（2.82 g/100 g per day） group,with a significant reduction in serum glucose（12.16 ± 1.00 mmol/L vs 17.65 ± 2.22 mmol/L,P 〈 0.01）,homeostasis model assessment of basal insulin resistance（22.68 ± 2.37 vs 38.79 ± 9.02,P 〈 0.05）,triglyceride（0.87 ± 0.15 mmol/L vs 1.99 ± 0.26 mmol/L,P 〈 0.01）,TC（3.31 ± 0.52 mmol/L vs 6.50 ± 1.04 mmol/L,P 〈 0.01） and LDL-c（2.47 ± 0.50 mmol/L vs 6.00 ± 1.07 mmol/L,P 〈 0.01）,and a signif icant increase in HDL-c（0.84 ± 0.08 mmol/L vs 0.50 ± 0.03 mmol/L,P 〈 0.01）.But the body weight was not changed signif icantly.CONCLUSION：YQZMT,which ameliorates insulin resistance and does not cause increase in body weight,may be a suitable therapeutic adjunct for the treatment of type 2 diabetes.
基金
Supported by The Fok Ying-Tong Education Foundation, China, No. 114036
Leading Academic Discipline Project of Shanghai Municipal Education Commission, No. J50307
State Administration of Traditional Chinese Medicine
