摘要
目的研究纯中药制剂不忘散对去卵巢(OVX)大鼠的血清雌激素水平和海马组织炎症反应的影响,探讨不忘散治疗阿尔茨海默病的作用机制。方法实验动物分为假手术(Sham)组、OVX组、17β-雌二醇(E2)组、中药不忘散高剂量(BH)组和中药不忘散低剂量(BL)组,每组各16只。连续给药12周后进行血清雌二醇的测定,RT-PCR检测海马组织芳香化酶(Arom)和胶质原纤维酸性蛋白(GFAP)mRNA表达,Western blot检测海马组织Arom和GFAP蛋白表达。结果 OVX组与Sham组、E2组比较血清雌二醇的含量明显降低(P<0.01),而BH组和BL组大鼠血清中雌二醇水平显著提高(P<0.01)。OVX组Arom mRNA、GFAP mRNA和蛋白表达较Sham组明显升高(P<0.01),BH组Arom mRNA表达较OVX组明显降低(P<0.01),E2组、BH组、BL组GFAP mRNA、Arom和GFAP蛋白表达与OVX组比较明显降低(P<0.01)。结论不忘散对OVX大鼠有益智作用,其机制可能与不忘散升高OVX大鼠血清雌二醇水平、维持星形胶质细胞的稳定、减少芳香化酶的表达、降低炎症反应有关。
Objective To study the effect of Bu-Wang-San（BWS） on estrogen and inflammatory responses in ovariectomized rats,and explore the mechanism of BWS in the treatment of Alzheimer′s disease.Methods The rats were divided into five groups： the Sham control group,the ovariectomized（OVX） group,the OVX＋ estradiol（E2） control group（OVX＋E2）,the high dosage of BWS group（OVX＋BH） and the low dosage of BWS group（OVX＋BL）,16 rats in each group.After administration for 12 consecutive weeks,the level of serum E2 was determined,mRNA expressions of aromatase（Arom） and glial fibrillary acid protein（GFAP） were analyzed by real-time PCR,and Arom and GFAP protein expressions were analyzed by Western blot.Results Level of serum E2 in the Sham group and the E2 group was significant higher than that in the OVX group（P0.01）.Treatment with BH and BL could statistically improve the level of serum E2（P0.01）.Expressions of Arom and GFAP mRNA and proteins in the OVX group were significantly higher than those in the Sham group（P0.01）.Expression of Arom mRNA in the BH group was statistically decreased compared with that in the OVX group（P0.01）.Expressions of GFAP mRNA and Arom and GFAP proteins in the E2 group,the BH group and the BL group were significantly lower than those in the OVX group（P0.01）.Conclusions BWS can ameliorate the cognitional function of OVX rats.The mechanism may be associated with increasing the level of serum estradiol,retaining the stability of astrocytes,reducing expression of Arom,and decreasing inflammatory responses.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第3期50-54,共5页
Journal of Shandong University:Health Sciences
基金
山东省医药卫生计划项目资助课题[2005(62)]
关键词
不忘散
去卵巢大鼠
雌激素
炎症反应
阿尔茨海默病
Bu-Wang-San
Ovariectomized rats
Estrogen
Inflammatory response
Alzheimer′s disease
