摘要
目的 探讨外周淋巴细胞可溶性白介素－２ 受体（ｓＩＬ－２Ｒ）释放与Ａｌｚｈｅｉｍ ｅｒ 病（ＡＤ）病情的关系。方法 流式细胞仪测定、ＭＴＴ 法和ＥＬＩＳＡ 法检测了刀豆蛋白Ａ（ＣｏｎＡ）和ＣＤ３ 单抗（ＯＫＴ３）刺激 ＡＤ 外周淋巴细胞白介素－２ 受体（ＩＬ－２Ｒ）表达、增殖和ｓＩＬ－２Ｒ 的释放。结果 （１）在ＣｏｎＡ 和ＯＫＴ３刺激下淋巴细胞增殖在轻、重ＡＤ 组均略下降，但刺激指数（ＳＩ）都大于２，与对照组无明显差异。（２）ｓＩＬ－２Ｒ 释放在轻、重度ＡＤ组均较对照组明显升高（Ｐ＜ ０．０５），ＩＬ－２Ｒ 的表达各组间无显著性差异。结论 ｓＩＬ－２Ｒ作为ＡＤ 的诊断、病情观察指标值得进一步探讨。
Objective To discuss the relationship between the expression of sIL 2R and the severity of Alzheimer’s disease stage. Methods The patients were divided into two groups mild and moderately severe according to severity of disease stage,and their lymphocytes were compared to those of elderly controls. Monoclonal antibodies labeled by FITC were used to cell membranal IL 2R expression analysis. Lymphocytes were stimulation with ConA and OKT 3,we observed the proliferative ability,cell membranal IL 2R expression and sIL 2R expression. Results (1)All groups revealed strong response to ConA and OKT 3,there were no significant differences between AD and controls. (2)The amount of sIL 2R revealed an increase in AD groups,there were significant differences between AD and controls(P<0.05). The expression of IL 2R revealed no significant differences between AD and controls. Conclusion Our results indicate that immunologic parameters may be useful in diagnosing and monitoring progression of AD.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
1999年第5期270-271,共2页
Journal of Apoplexy and Nervous Diseases
关键词
SIL-2R
早老性痴呆
T细胞
活化
细胞增殖
Alzheimer’s disease Lymphocyte Soluble interleukin 2 receptor Proliferative ability
