摘要
红三叶草(Trifolium pratense L.)离体根在改良的White培养基上能连续继代培养。将发芽的中国球囊霉(Glomus sinensis Peng & Shen)孢子接种三叶草离体根,在以 CaHPO_4为磷源的 White 培养基上培养,能稳定地形成泡囊-丛枝状内生菌根(Vesicular-ArbuscularMycorrhizae,VAM)。真菌在侵染前,菌丝在根分泌物的刺激下常进一步伸长,当菌丝接触到根的适当部位后即开始侵染,侵入前常首先形成附着胞,并通过附着胞进一步向根内侵染,形成具有根内菌丝、丛枝和不明显泡囊的内生菌根。随后产生根外菌丝,并产生少量薄壁的'无性孢子'。这些'无性孢子'不能发育成正常的厚垣孢子。本文讨论了这一技术在研究 VA 菌根中的作用和意义。
The excised roots of red clover(Trifo-lium pratense)were subcultured in the modi-fied White's medium.Vesicular-arbuscularmycorrhizae(VAM)were established in theexcised roots inoculated with the germinatedspores of Glomus sinensis in the modifiedWhite's medium containing CaHPO_4.Thegerm tubes of spores usually elongated fur-ther under the stimulation of growing rootsafter being inoculated.Prior to infection,the fungal hyphae first contacted the growingroot and then produced appressoria withwhich the fungal hyphae entered the cortex ofthe root,and formed VA mycorrhizae witharbuscules and unclear vesicules.Finallymany external mycelium and small numberof 'vegetative spores' appeared in the me-dium.The 'vegetative spores' were unable todevelop into normal chlamydospores in thepresent conditions.The significance and im-plication of the results in the study of VAMwere discussed.
出处
《微生物学报》
CAS
CSCD
北大核心
1990年第5期375-379,共5页
Acta Microbiologica Sinica
关键词
三叶草
离体根
中国球囊霉
VA菌根
Trifolium pratense
Excised root
Glomus sinensis
VA mycorrhizae
