摘要
分别在酸性和碱性介质研究了偶氮胂Ⅲ，甲基蓝，溴酚蓝，酸性品红和亚甲基蓝同蛋白质结合的光度性质．在适宜ｐ Ｈ 条件下，上述染料均可与蛋白质结合，使染料吸光度下降．除亚甲基蓝外，其余染料吸光度的降低与一定浓度范围的蛋白质成正比．初步讨论了染料性质与蛋白质结合程度的关系．
Spectrophoto metric characters of protein com bination with Arsenazo Ⅲ, Methyl blue , Bro mophenol blue , Fuchsine acid and Methylene blue have been studied in acidic mediu m and alkalinemediu m respectively . Protein can co m bine the above mentioned dye to reduce absorptivity of dye so lution at optim um p H. The concentration of protein in a given range is proportional with reducedabsorptivity of above dye solutions except for Methylene blue . The m utual relation of dye propertiesand ability to com bine protein have been discussed initially .
出处
《广东工业大学学报》
CAS
1999年第3期102-105,共4页
Journal of Guangdong University of Technology
