摘要
将现有的抗草甘膦基因(sxglr-11)和带有Ubiquitin启动子的载体进行连接,用壮观霉素(spectino-mycin)抗生素标记,构建携带抗草甘膦基因(sxglr-11)的大肠杆菌。然后利用三亲杂交法,将构建的真核表达载体转入根瘤农杆菌菌株LBA4404。对菌落进行PCR检测以及质粒双酶切产物进行电泳检测,并用构建的工程菌转化玉米试管苗。研究结果表明,表达载体成功转入根瘤农杆菌LBA4404。
Glyphosate-resistant gene existing （sxglr-11 ）and vector carrying the Uhiquitin promoter were connected and marked with speetinomycin （spectinomycin） antibiotic marker, to construct gene carrying glyphosate （ sxglr- 11 ） in the coliform bacilli. Later, with three-parental mating method, the eukaryotic expression vector transferred into Agrobacterium tumefaciens strain LBA4404. The products of mycelium PCR, plasmid PCR and plasmid digested by EcoR I and BamH I enzyme were tested by electrophoresis, and the constructed project Agrobacterium was cultured with plantlet of maize. The result showed that eukaryotic expression vector had been transformed into A grob acte rium tumefacie ns LBA4404.
出处
《山西农业科学》
2011年第5期389-391,407,共4页
Journal of Shanxi Agricultural Sciences
基金
山西省青年基金项目(2009021032-2)
山西省农业科学院科技攻关项目(YGG0849)
关键词
玉米
真核表达载体
根癌农杆菌
抗草甘膦基因
maize
eukaryotic expression vector
Agrobacterium tumefaciens
anti-glyphasate gene
