摘要
采用聚丙烯酰胺凝胶电泳法对10个玉米品种进行酯酶同工酶分析。结果表明,各品种在酶谱上有丰富的表达,并呈现很强的特异性,且在多个位点上出现稳定一致的基本酶带,供试材料共出现76条酶带,其数量在品种间差异显著,其中最多出现10条,最少5条。其中一部分品种酶带位点相似,但酶带的染色深浅不同形成了各个品种特有的谱带。在亲缘关系方面通过计算各品种的酶谱联合系数发现,赣新花糯一号与台湾超甜38号的亲缘关系最远,豫禾988和渝彩甜糯一号的亲缘关系较近,其中洛阳800、沈单16和鲁单981之间的亲缘关系最近。
Esterase isozymes of different maize varieties were analyzed by polyacrylamide gel electrophoresis.The results showed rich expression and strong specificity in enzyme spectrums of all the varieties,and there were stable and consistent bands of basic enzymes in many alleles.There were 76 enzyme bands in total for experiment materials.The numbers were significantly different between varieties,10 for the largest and 5 for the least.Some varieties possessed similar enzyme sites,but there were specific enzyme bands in various types due to different degree of staining.In the relationships,Ganxinhuanuo No.1 and Taiwanchaotian No.38 were the farthest,Yuhe 988 and Yucaitiannuo No.1 were closer,and Luoyang 800,Shendan 16 and Ludan 981 were the nearest based on calculation of zymography combined coefficient.
出处
《河南农业科学》
CSCD
北大核心
2011年第3期32-34,104,共4页
Journal of Henan Agricultural Sciences
基金
河南科技大学人才科学研究基金(09001321)
