摘要
首次在果树作物上使用cDNA-RAPD技术,以藤稔葡萄正常成花花芽和摘心处理后成花花芽为材料,分析了摘心处理后与葡萄夏芽成花相关基因的差异表达。通过10条RAPD引物分析,在基因组和cDNA上共获得153条(差异性条带81条)扩增片段,并进一步将这些片段分为3种类型。摘心处理后的葡萄夏芽在成花过程中部分基因出现了不同程度的差异表达,且差异表达的基因大多数呈现表达增强的趋势。对差异性片段进行选择回收和测序,共获得14条核苷酸片段,其中有13个片段能在GenBank数据库中发现相应的同源序列。说明该技术是一种较好的可用来研究基因差异表达的方法。
In order to initiate some investigation of the molecular mechanism of flowering in grapevine,differential expression profile of cDNA between the summer buds on the shoots after cut back and those of CK was analyzed by cDNA-RAPD.Ten pairs of primer were screened used for the cDNA-RAPD amplification.Totally,153（81 differentially expressed fragments）fragments were detected from the grapevine genomic DNA-and cDNA-RAPD fingerprints that could be classed into 3 types.Gene expression of the treatments had some change,and the majority of differentially expressed genes showed increased expression.Fragments with obvious difference were selected to re-amplification and purification,sequenced,and 14 sequences were obtained.Most of them could be blasted at various levels with the sequence deposited in GenBank database.It showed that the cDNA-RAPD technology can be an preferential method for study on genes′differential expression.
出处
《华北农学报》
CSCD
北大核心
2011年第2期43-48,共6页
Acta Agriculturae Boreali-Sinica
基金
中央高校基本科研业务费专项资金(KYJ200909)
江苏省科技支撑项目(BE2010326)
