摘要
目的:进行穴位移植与静脉移植两种不同移植途径来观察兔异体骨髓间充质干细胞(MSCs)对梗死区CD31及bFGF表达的影响的研究。方法:将已建立心肌梗死模型的日本大耳白兔随机分为3组,包括模型对照组、穴位注射干细胞组、静脉注射干细胞组。用开胸结扎冠状动脉前降支的方法建立急性心肌梗死模型,进行间充质干细胞分离、培养、传代、流式细胞仪检侧兔MSCs表面标志、收集、BrdU标记、Brdu标记率的检测,利用免疫组化法检测。结果:梗死心肌组织中CD31平均光密度值,与正常组相比较,穴位注射干细胞组均降低,P<0.01,静脉注射干细胞组略升高,P>0.05;与模型对照组比较,穴位注射组P<0.05,静脉注射组P<0.01;穴位注射组与静脉注射组比较,P=0.05。梗死心肌组织中BFGF平均光密度值,与正常组相比较,穴位注射干细胞组升高,P<0.05,静脉注射干细胞组降低,P<0.01;与模型对照组比较,穴位注射组P<0.05,静脉注射组P<0.01;穴位注射组与静脉注射组比较,P<0.01。结论:穴位与静脉注射骨髓间充质干细胞显著促进了梗死心肌组织周围内皮细胞的激活、增殖、迁移,显著改善梗死区血液循环。但与穴位组相比较静脉组CD31没有显著差异。穴位注射骨髓间充质干细胞显著促进了梗死心肌组织中表达促毛细血管新生细胞因子bFGF。
Abstract：Objective：To investigate the affect of that how bone marrow stromal cells （MSCs） work in infracted region with CD31, bFGF through two ways, point injection and intravenous injection. Methods：Models of acute myocardial, in- farction on big - eared Japanese rabbits were divided into three groups which are model control group, point injection stem cell group and intravenous injection of stem cell group. Models of acute myocardial infarction were established by ligating left anterior descending coronary artery on rabbits. MSCs were isolated,cultivated,subcuhured,tested with MSCs surface- marker by Flow Cytometer, collected, labeled with Brdu, tested with labeling rate of Brdu, and finally tested by immuno- histochemical method. Results：Compared with normal group ,The mean OD of CD31 in infracted region in point injection stem cell group were low（ P 〈 0.01 ） ,intravenous injection of stem cell group were high（P 〉 0.05 ） ;Compared with con- trol group, point injection stem cell group were P 〈 0.01, intravenous injection of stem cell group were P 〉 0.05 ; Com- pare intravenous injection of stem cell group with point injection stem cell group, and they were same （P = 0.05 ） ; Com- pared with normal group, the mean OD of BFGF in infracted region in model point injection stem cell group was high （P 〈 0.05 ）, and intravenous injection of stem cell group was low（ P 〈 0. O1 ） ;Compared with control group, point injection stem cell group were P 〈 0.05, and intravenous injection of stem cell group were P 〈 0.01. Compared with point injection stem cell group, intravenous injection of stem cell group was low（ P 〈 0.01 ）. Conclusion：There is significance that Stem cell injected into points and veins can activate, proliferate, transfer and improve blood circulation in infracted region. Compared with point injection stem cell group,these is no significant difference of CD 31 in intravenous injection of stem cell group. Expression of angiogenesis cell factor bFGF is increased in infracted region by point injection with stem cell.
出处
《中华中医药学刊》
CAS
2011年第5期1073-1076,共4页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅科技攻关项目(2004D281)
辽宁省教育厅创新团队项目(2007T120)
