摘要
采用聚合酶链反应（ＰＣＲ）对８４ 例动脉粥样硬化性脑梗塞（ＡＣＩ）患者（ＡＣＩ组）的载脂蛋白Ｂ（ａｐｏＢ）基因３′端小卫星区（ＭＳＲ）的ＤＮＡ多态性进行研究，并与１０７ 例正常人（对照组）进行比较。结果两组ａｐｏＢ基因３′端等位基因分布频率均以ＭＳＲ３７ 和ＭＳＲ３９ 最高，ＡＣＩ组分别为０．４８、０．１９，对照组分别为０．５０、０．１７，且ＡＣＩ组的大拷贝等位基因（ＭＳＲＢ）分布频率增高。组内及组间分析显示，ＭＳＲＢ与血清ａｐｏＢ以及ＬＤＬ－ｃｈ 增高有关；３′端ＭＳＲ等位基因是已知ａｐｏＢ基因的有用标记；大片段ＭＳＲ等位基因与ＡＣＩ有相关性，支持不同拷贝数目的基因多态性可影响血脂水平代谢；位于ａｐｏＢ３′端的ＭＳＲ等位基因的多态性，可能是动脉粥样硬化多源性病因的一部分，其可能涉及病理过程中胶原暴露及内皮损伤，并共同参与ＡＣＩ的发病。
The DNA polymorphisms in ministellite region at 3′ end of apolipoprotein B(apoB)gene of 84 patients with atherosclerotic cerebral infarction(ACI)and 107 normal healthy controls were observeds by polymerase chain reaction technique Results showed that The frequency distribution of apoB 3′ministellite region alleles were in monomold peaks at 37 and 39 repeat uints in both group,the big number repeat units(MSRB)was higher in ACI than normal controls The homozygote genotype of the big number repeat units alleles had a relationship to the high level of apoB and LDL ch There were same tandency in ACI and controls This suggests the possibility that there apoB 3′gene ministellite region alleles polymorphism increase the susceptibility to atherosclerosis by means other than by altering lipoprotein levels desverves further consideration
出处
《山东医药》
CAS
北大核心
1999年第19期11-12,共2页
Shandong Medical Journal
