摘要
为了建立苹果砧木中砧1号茎尖离体保存技术,以M9和富士为对照,研究了培养基中添加不同浓度蔗糖或生长抑制剂对试管苗生长速率的影响以及包埋干燥法和玻璃化法预处理对试管苗茎尖超低温保存效果的影响。结果表明,不添加生长抑制剂,蔗糖浓度为20 g·L-1时,中砧1号试管苗离体保存效果比较好。常规培养基(蔗糖浓度30 g·L-1)中添加5 mg·L-1 CCC、0.1 mg·L-1 ABA或60 mg·L-1 B9,也可以达到相同的效果。用包埋干燥法和PVS3玻璃化液预处理,中砧1号超低温保存后的茎尖再生率较高。综合上述结果,中砧1号低温保存的适宜条件为培养基中蔗糖浓度20 g·L-1;超低温保存的适宜条件为包埋干燥法预培养蔗糖浓度0.3 mol·L-1,包埋干燥时间4 h。
In order to establish in vitro conservation techniques of shoot tips of apple rootstock Zhongzhen1,the experiment probed the influences of culture media supplemented with various concentration of sucrose or growth inhibitors respectively on the growth rate of the test-tube seedlings.Meanwhile,the effects of encapsulation-dehydration and vitrification pretreatment on in vitro shoot tips were researched in Zhongzhen1,compared with M9 and Fuji.The results were as follows： the effect of conservation was better when seedlings grew in culture media with 20 g·L-1 sucrose and no inhibitors,the same result appeared in normal medium（30 g·L-1 sucrose） supplemented with 5 mg·L-1 CCC,0.1 mg·L-1 ABA or 60 mg·L-1 B9.The regeneration rate of shoot tips of zhongzhen1 was high after encapsulation-dehydration and PVS3 vitrification solution pretreatment.Hypothermic conservation results suggested that the optimum concentration of sucrose in tissue culture was 20 g·L-1,and cryopreservation results showed that encapsulation-dehydration was better than vitrification,the optimum concentration of sucrose in preculture was 0.3 mol·L-1,and the optimum encapsulation time was 4 h.
出处
《果树学报》
CAS
CSCD
北大核心
2011年第3期498-502,共5页
Journal of Fruit Science
基金
国家转基因专项(2009ZX08009-122B)
行业科技项目(200903044)
中央高校基本科研业务费专项(2009-2-06)
关键词
苹果
砧木
离体保存
超低温保存
Apple
Rootstock
In vitro conservation
Cryopreservation
