摘要
从辽宁某猪场采取病料,经处理后接种Marc-145细胞进行PRRSV分离,结果有2份病料在该细胞上盲传后可见典型的CPE,经测定其毒价为10-6.5TCID50/mL。间接免疫荧光试验可见黄色荧光,用电镜对纯化的病毒进行观察,可见有囊膜包裹的圆形病毒粒子,直径约为50～70 nm。该病毒对氯仿、紫外线、酸碱度变化和热敏感。对有CPE的细胞培养物进行RT-PCR,结果为阳性。接种试验动物出现典型的PRRS临床症状和死亡。结果表明,成功分离到一株PRRSV。
To isolate porcine reproductive and respiratory syndrome virus（PRRSV）,the morbid materials were collected from some swine farm of Liaoning province and inoculated on Marc-145 cell after disposal.After blind transfer of culture,two portions showed typical cytopathic effect（CPE）.TCID50 of virus was 10-6.5/mL.By the indirect immunofluorescence experiment,the yellow green fluorescence occurred in the cell cytoblastema.The virus appeared typical porcine reproductive and respiratory syndrome virus form： diameter was about 50-70 nm,circle with peplos and peplomer.It was inactivated by chloroform,ultra-violet,pH variation,and heat.The cell cytoblastema which showed CPE were tested by RT-PCR showed positive.The laboratory animals appeared obvious clinical symptom and death after inoculation.The result showed that PRRSV was identified from Liaoning province.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第5期173-176,共4页
China Animal Husbandry & Veterinary Medicine
关键词
猪繁殖与呼吸综合征病毒
分离
鉴定
porcine reproductive and respiratory syndrome virus（PRRSV）
isolation
identification
