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中国沿海养殖牡蛎尼氏单孢子虫感染情况调查 被引量:2

Investigation of Haplosporidium nelsoni Infection in Breeding Oyster of China Bays
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摘要 单孢子虫病是严重危害世界贝类养殖业的主要疫病之一。本研究选取中国沿海养殖的牡蛎作为研究对象,采用OIE推荐的PCR方法结合测序技术,对采自中国沿海4个监测点养殖牡蛎的感染情况进行调查,结果表明,这些监测点养殖的牡蛎均不同程度感染了单孢子虫。经PCR测序及序列分析表明感染种类均为尼尔森单孢子虫(Haplosporidium nelsoni)。 Haplosporidiosis is a serious mollusc disease caused by parasitic Haplosporidium. The Haplosporidium-specific PCR detecting method, recommanded by World Animal Health Organization (OIE), was adopted to investigate Haplosporidiosis prevalence of oyster in three sampling sites of China east bays. Results showed that there was Haplosporidium prevalence in all the four sites in different degree. Through combining with DNA sequencing and sequence analysis, the infection species was identified to he Haplosporidium nelsoni.
出处 《中国畜牧兽医》 CAS 北大核心 2011年第5期158-160,共3页 China Animal Husbandry & Veterinary Medicine
基金 质检公益专项(201010016) 国家质检总局科研基金(2007IK038)资助
关键词 单孢子虫 PCR 测序 感染情况调查 序列分析 Haplosporidium PCR sequencing prevalence sequence analysis
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参考文献10

  • 1王中卫,吕昕,梁斌,梁玉波.长牡蛎尼氏单孢子虫的初步研究[J].海洋环境科学,2009,28(2):164-166. 被引量:11
  • 2谢丽基,谢芝勋,庞耀珊,刘加波,邓显文,谢志勤.贝类单孢子虫PCR检测方法的建立[J].中国畜牧兽医,2010,37(1):54-56. 被引量:7
  • 3谢丽基,谢芝勋,庞耀珊,刘加波,邓显文,谢志勤.贝类单孢子虫荧光定量PCR检测方法的建立[J].西南农业学报,2010,23(1):239-242. 被引量:7
  • 4Andrews J D,Wood J L.Oyster mortality studies in Virginia. Ⅵ. History and distribution of Minchinia nelsoni, a pathogen of oysters in Virginia[J]. Chesapeake Sci,1967,8:1-13.
  • 5Burreson E M,Ford S E. A review of recent information on the Haplosporidia, with special reference to Haplosporidium nelsoni (MSX disease)[J].Aquat Living Resour, 2004,17: 499-517.
  • 6Ford S E,Haskin H H. History and epizootiology of Haplosporidium nelsoni (MSX), an oyster pathogen in Delaware Bay,1957-1980[J]. Journal of Invertebrate Pathology,1982,40:118-141.
  • 7Friedman C S. Haplosporidian infections of the Pacific oyster Crassostrea gigas(Thunberg), in California and Japan[J]. Shellfish Res,1996,15:597-600.
  • 8Nancy A S,Mark E S,Eugene M B.Detection of Haplosporidium nelsoni (Haplosporidia: Haplosporidiidae) in oysters by PCR amplification[J].Dis Aquat Org,1995,23: 145-152.
  • 9Sunila I,Karolus J,Volk J. A new epizootic of Haplosporidium nelsoni(MSX), a haplosporidian oyster parasite, in long island sound, Connecticut[J]. Shellfish Res,1999,18:169-174.
  • 10Stephenson M F,McGladdery S E,Maillet M V,et al. First reported occurrence of MSX in Canada[J]. Shellfish Res,2003,22:355.

二级参考文献34

  • 1BURRESON E M, FORD S E. A review of recent information on the Haplosporidia, with special reference to Haplosporidium nelsoni (MSX disease) [ J]. Aquat. Living Resour, 2004, 17:499- 517.
  • 2NEWELL RIE, BARBER B J. A physiological approach to the study of bivalve molluscan diseases [ J]. Am Fish Soc Spec Publ, 1988, 18:269-280.
  • 3HASKIN H H, STAUBER L A, MACKIN J A. Minchinia nelsonin. sp. ( Haplosporida, Haplosporidiidae) : Causative agent of the Delaware Bay oyster epizootic [ J]. Science, 1966, 153 : 1414-1416.
  • 4KERN F G. Sporulation of Minchinia sp. ( Haplospofida, Haplosporidiidae) in the Pacific oyster Crassostrea gigas (Thunberg) from the Republic of Korea [ J ]. Protozool, 1976, 23:498-500.
  • 5FORD S E. Effect of repeated hemolymph sampling on growth, mortality, hemolymph protein and parasitism of oysters, Crassostrea virginica[ J]. Physiol, 1986, 85A:465-470.
  • 6FORD S E, KANALEY S A, MICHAEL F, et al. "Panning" ,a technique for enrichment of the parasite Haplosporidium nelsoni (MSX) from hemolymph of infected oysters[ J]. Journal of Invertebrate Pathology, 1990, 56:347-352.
  • 7TRISTAN R, STOKES N A, BRUNO C, et al. Haplosporidiosis in the Pacific oyster Crassostrea gigas from the French Atlantic coast[ J]. Disease of Aquatic Organisms, 2000, 42:207 214.
  • 8STOKES N A, BURRESON E M. A sensitive and specific DNA probe for the oyster pathogen Haplosporidium nelsoni [ J ]. J Eukaryot Microbiol, 1995, 42:350-357.
  • 9MICHAEL D J, DEAN E, FRANKLIN et al. Use of competitive PCR to detect and quantify Haplosporidium nelsoni infection (MSX) in the eastern oyster ( Crassostrea virginica) [ J]. Mar. Biotechnol, 2000, 2:456-465.
  • 10TAKASHI K, TOMOYOSHI Y. Detection of Haplosporidium nelsoni in Pacific oyster Crassostrea gigas in Japan [ J ]. Fish Pathology, 2002, 37 (4) : 193-195.

共引文献14

同被引文献26

  • 1全为民,沈新强,罗民波,陈亚瞿.河口地区牡蛎礁的生态功能及恢复措施[J].生态学杂志,2006,25(10):1234-1239. 被引量:52
  • 2邵炳绪,唐子英,孙帼英,邱郁春,邵贻钧,薛镇宇.松江鲈鱼繁殖习性的调查研究[J].水产学报,1980,4(1):81-86. 被引量:38
  • 3王中卫,梁玉波,梁斌,吕昕.海产贝类尼氏单孢子虫病害的研究进展[J].现代生物医学进展,2006,6(12):123-126. 被引量:1
  • 4全为民,张锦平,平仙隐,施利燕,李培军,陈亚瞿.巨牡蛎对长江口环境的净化功能及其生态服务价值[J].应用生态学报,2007,18(4):871-876. 被引量:41
  • 5Andrews, J. D., and Wood, J. L. 1967. Oyster mortality studies in Virginia. VL. History and distribution of Minchinia nelsoni., a pathogen of oysters, in Virginia. Ches. Sci. 8(1) :1-13.
  • 6Tschentscher, F., Ulrich, H., Frey, U. H., and Bajanowski, T. 2008. Amelogenin sex determination by pyrosequencing of short PCR products. Int. J. Legal Med. 122(4) :333-335.
  • 7Haskin, H. H., Stauber, L. A., and Mackin, J. A. 1996. Minchinia nelsonin, sp. ( Haplosporidia, Haplosporidiidae) : causative agent of t he Delaware Bay oyster epizootic. Science, 153 : 1 41- 1 416.
  • 8Hillman,R. E.,Boehm,P. D.,and Freitas,S. Y. 1988. A pathology potpourri from the NOAA mussel watch program. J. Shellfish Res. 7:216-217.
  • 9Kern, F. G. 1988. Recent changes in the range of "MSX" Haplosporidium nelsoni. J. Shellfish Res. 7,543-544.
  • 10Matthiessen,G. C.,Feng,S. Y.,and Leibovitz, L. 1990. Patterns of MSX(Haolosporidium nelsoni) infection and subsequent mortality in resistant and susceptible strains o5 the eastern oyster Crassostrea virginica (Gmetin, 1791) in New England. J. Shellfish Res. 9:359-366.

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