摘要
从广东地区发病鸭中分离到1株新城疫病毒,血凝试验、血凝抑制试验结果表明此分离株具有血凝活性,且这种血凝性可被NDV标准阳性血清抑制,而不能被AIV-H5、AIV-H7、AIV-H9、EDSV阳性血清抑制;用针对NDVF基因特异性鉴定引物对该分离株进行聚合酶链反应扩增,可扩增出相应的目的片段。由此,初步鉴定该分离株为鸭源新城疫病毒,并命名为NDV/DUCK/SD/2006。另外,通过毒力测定,该毒株的MDT、IVPI分别为51.75 h、2.44,判定为强毒力株。
A Newcastle disease virus（NDV） was isolated from duck in Guangdong.It was identified with hemagglutination,hemagglutination inhibition.These results showed the isolate had hemagglutination and was inhibited by the standard antibody of AIV-H5,AIV-H7,AIV-H9 and EDSV.It was amplified by RT-PCR and sequenced,which was used by the specific identification primers of NDV F gene.Thus,the isolate was identified as a duck NDV,named NDV/DUCK/SD/2006.In this research,the isolate were conducted virulence determination.Results showed that,the MDT,and IVPI of the isolate were 51.75 h,2.44 respectively,suggesting it was a velogenic strain.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第5期143-145,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31072319)
公益性行业(农业)科研专项经费(200803020-08)
关键词
新城疫病毒
鸭
广东
生物学特性
NDV
duck
Guangdong
biological characterization
