摘要
为了分析猪链球菌2型(SS2)发病原因、流行趋势,为预防和控制猪链球菌2型提供理论依据,根据GenBank发表的猪链球菌精氨酸脱亚氨酸酶(arginine dei minase,ADS)基因序列,设计并合成引物,采用RT-PCR技术,对阳性病料进行扩增,回收扩增产物并将其克隆入pMD18-T载体中,提取质粒测序。应用DNAStar软件分析核苷酸序列的同源性,并绘制系统进化树。结果显示,分离株HN1-HN8核苷酸的同源性为94.9%～100%,来自同一地区的分离株HN5和HN6同源性为100%,而河南分离株与GenBank发表的SX332同源性为95.9%～99.2%。因此,同一地域分离株间ADS基因序列同源性较高且亲缘关系较近,不同地域则较远。
The aim was to analyze the reason and epidemic trend of Streptococcus suis serotype 2,and provide theoretical basis for preventing and controlling it.According to the sequence of arginine deiminase gene published by the Genbank,The primers was designed for RT-PCR amplification of ADS isolates from Henan obtaining its DNA fragments,which were cloned into pMD18-T vector respectively and sequenced.The sequences of ADS genes were analysed by DNAStar,analysed nucleotide sequence homology and drawed phylogenetic tree.The nuclear acid analysis indicated that the homology was 94.9% to 100% between the isolation strains,the homology was 100% between HN5 and HN6 from the same region,and the homology of nuclear acid sequence was 95.9% to 99.2% between Henan isolation strains and SX332 issued by the GenBank.The homology of ADS gene sequence was higher and genetic relationship was near during prevalence strains in the same region,and it was far in different regions.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第5期117-120,共4页
China Animal Husbandry & Veterinary Medicine
基金
河南城建学院基金项目(2010JYB011)
