摘要
目的 观察２ 例慢性 Ｈ Ｃ Ｖ 携带者及１ 例 Ｈ Ｃ Ｖ Ｒ Ｎ Ａ 转阴者体内包含 Ｃ Ｔ Ｌ 抗原表位的 Ｈ Ｃ Ｖ Ｎ Ｓ３ 区部分区段的长期演变。方法 通过反转录 Ｐ Ｃ Ｒ 扩增， Ｍ１３ 亚克隆，对３ 例 Ｈ Ｃ Ｖ 感染者的 Ｈ Ｃ Ｖ Ｎ Ｓ３ 区部分区段的一级结构进行测定。对感染者的 Ｈ Ｌ Ａ 进行分型。根据基序（ ｍｏｔｉｆ） 及 Ｈ Ｌ Ａ 分型资料预测该区段中的 Ｃ Ｔ Ｌ 抗原表位。结果 文献报道的 Ｈ Ｌ Ａ Ａ２ 限制的抗原表位在无 Ｈ Ｌ Ａ Ａ２ 的感染者 Ｃ 中，１９９１ 年至１９９６ 年氨基酸序列无变异。具有 Ｈ Ｌ Ａ Ａ２ 的感染者 Ｗ， Ｚ 部分氨基酸序列中该表位的起始密码子发生无义突变，测序资料中包含稳定的变异位点的肽段，符合 Ｍ Ｈ Ｃ结合肽的基序（ ｍｏｔｉｆ） 。结论 无义突变可能与 Ｃ Ｔ Ｌ 的免疫压力有关。稳定变异位点产生的原因可能是免疫逃避。
Longterm evolution of the region NS3 containing a CTL epitope of hepatitis C virus in three HCV infected men$$$$ GUO Huazhang, WANG Wenliang, WANG Tao, et al. Department of Pathology, Fourth Military Medical University, Xi'an 710032 【 Abstract 】 Objective To study the sequence evolution of HCV NS3 in two HCV carriers and one recovered person over 6 years. Methods Three patients were exposed to HCV prior to 1991. Blood samples taken in 1991, 1994 and 1996 were analyzed for nucleotide sequences. HLA types of these three persons were determined using antibodies. CTL epitopes were predicted using MHC binding motif. Results A reported HLA A2 restricted CTL epitope showed no alteration in the HLA A2 negative carrier over six years. In the HLA A2 positive individuals, some of the sequences showed amber mutation on the initial codon of the epitope. Peptides with persistent amino acid transitions contained MHC binding peptide motif. Conclusion The amber mutations and persistent amino acid transitions may be related to the immune escape. 【 Subject words 】 Hepatitis C virus Tlymphocytes Viral protein
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第5期372-375,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
