摘要
以牛血清白蛋白为模板分子,四氧化三铁磁性纳米粒子为载体,多巴胺为功能单体制备磁纳米分子印迹聚合物。用制备的磁纳米分子印迹聚合物吸附蛋白,并测定对蛋白的吸附容量。结果表明:随着吸附时间的增加,吸附量增加,一段时间后可达到吸附饱和;在其他条件不变的情况下,模板浓度增加,吸附容量相应增加;印迹聚合物及其非印迹聚合物对牛血清白蛋白、人血红蛋白和牛血红蛋白的选择性吸附表明所制备的磁性分子印迹聚合物对牛血清白蛋白具有特异性吸附效果。
A molecular imprinted polymer was prepared using bovine serum albumin as the template molecule,iron oxide magnetic nanoparticles as the carriers and dopamine as the functional monomer.The prepared magnetic molecularly imprinted polymer nanoparticles（MNP-MIPs） were applied to adsorb protein and the protein adsorption capacity was measured.The results indicated that the adsorption capacity towards protein exhibited an increase up to the saturation level with prolonged adsorption time.Similarly,the adsorption capacity also revealed an increase trend as the template concentration rose and other conditions were kept unchanged.Moreover,bovine serum albumin could be specifically adsorbed by MNP-MIPs.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第10期61-65,共5页
Food Science
关键词
分子印迹
磁性纳米粒子
多巴胺
molecular imprinting
magnetic nanoparticle
dopamine
