摘要
为研究牛甘露糖结合凝集素1(mannose-binding lectin 1,MBL1)基因启动子区单核苷酸多态性,采用聚合酶链式反应限制片长多态性(restriction fragment length polymorphism,RFLP)、单链构象多态性(single-strand conformation polymorphism,SSCP)及直接测序的方法检测了1 073头中国荷斯坦奶牛、69头鲁西黄牛和24头渤海黑牛启动子区-2194(A>C)、-1446(T>C)、-1330(G>A)三个位点的不同基因型分布。结果表明,在-2194位点和-1330位点处渤海黑牛只检测到两种基因型(AA/AC,GG/GA);而鲁西黄牛群体中在-1330位点处没有检测到AA型。三个品种牛群在该三个位点的优势等位基因相同,分别为A、C、G,频率为A(CH 0.900 1/LYC 0.760 9/BBC 0.937 5)、C(CH 0.604 8/LYC 0.768 1/BBC 0.625)和(CH 0.725 1/LYC0.869 6/BBC 0.895 8)。三个品种牛的优势等位基因相对保守,需进一步研究分析这3个多态位点与牛生产性状的关系。
In order to study the single nucleotide polymorphisms（SNPs） in the promoter region of Mannose-binding Lectin1,the genotypes of 3 loci at-2194（AC）,-1446（TC）,-1330（GA） in the promoter region of MBL1 were detected by RFLP（restriction fragment length polymorphism）,SSCP（single-strand conformation polymorphism） and directly sequencing method,with 1 073 Chinese Holstein cattles,69 Luxi Yellow cattle and 24 Bohai Black cattles as materials.The results showed that only 2 genotypes（AA/AC,GG/GA） were observed at sites-2194（AC）and-1330（GA）in Bohai Black cattle,and in Luxi Yellow cattles,the genotype AA was not detected at site-1330（GA）.The dominant alleles in 3 breeds were A,C and G,and the allelic frequencies were A（CH 0.900 1/LYC 0.760 9/BBC 0.937 5）、C（CH 0.604 8/LYC 0.768 1/BBC 0.625） and G（CH 0.725 1/LYC 0.869 6/BBC 0.895 8）,respectively.The above results indicated that among all detected loci,the dominant alleles in 3 breeds were conservative relatively.Thus the 3 SNPs association with production traits needs further studies.
出处
《中国农业科技导报》
CAS
CSCD
2011年第2期38-45,110,共8页
Journal of Agricultural Science and Technology
基金
国家863计划项目(2006AA10Z1D92007AA10Z169)
现代农业产业技术体系(nycytx-0107)
山东省良种工程项目(2007LZ015-06)
山东省农业科学院创新基金(2006YCX028)资助
关键词
牛
MBL1基因
启动子区
单核苷酸多态性
Bos taurus
MBL1 gene
promoter region
single nucleotide polymorphisms（SNPs）
