摘要
目的:探讨雌二醇联合睾丸酮对乳腺癌MCF-7细胞增殖和凋亡的影响及其可能机制。方法:将10-10mol/L雌二醇和10-5、10-7、10-9和10-11mol/L睾丸酮单独或联合作用于乳腺癌MCF-7细胞24、48和72h,MTT法检测细胞的生长情况,FCM检测细胞周期和细胞凋亡的情况,以及cyclinD1和雄激素受体(androgen receptor,AR)蛋白的表达情况。结果:雌二醇可促进MCF-7细胞的增殖。高浓度(10-5mol/L)睾丸酮可抑制细胞增殖,并抑制雌二醇促细胞增殖的作用;低浓度(10-9mol/L)睾丸酮可促进MCF-7细胞增殖,并增强雌二醇的促细胞增殖作用。雌二醇联合10-5mol/L睾丸酮作用48h后促使细胞周期由G1期进入S期,细胞凋亡率上升,cyclinD1蛋白的表达上调,AR蛋白的表达未见明显变化。结论:雌二醇联合高浓度睾丸酮可抑制乳腺癌细胞的增殖和促进细胞凋亡,可能与上调细胞cyclinD1蛋白的表达有关。
Objective：To study the effects of estradiol plus testosterone on the proliferation and apoptosis of breast cancer cell line MCF-7,and to elucidate the possible mechanism.Methods：The growth of MCF-7 cells treated with estradiol（10-10 mol/L） or testosterone（10-5,10-7,10-9 and 10-11 mol/L） alone or in combination for 24,48 and 72 h was detected by MTT assay.The cell cycle distribution and the apoptosis rate of MCF-7 cells were determined by flow cytometry（FCM）.The expression levels of cyclinD1 and androgen receptor（AR） proteins were examined by FCM.Results：The proliferation ability of MCF-7 cells was elevated after treatment with estradiol,and this effect could be inhibited by a higher concentration of testosterone（10-5 mol/L） while improved by a lower concentration of testosterone（10-9 mol/L）.After treatment with estradiol plus testosterone（10-5 mol/L） for 48 h,the G1/S phase transition of MCF-7 cells was accelerated,and the apoptosis rate was increased;the expression level of cyclinD1 protein was increased while no change of AR protein expression was observed.Conclusion：Estradiol combined with testosterone of high concentration can inhibit the proliferation of MCF-7 cells and improve the apoptosis.This effect may be associated with the up-regulation of cyclinD1 expression.
出处
《肿瘤》
CAS
CSCD
北大核心
2011年第3期192-196,共5页
Tumor
基金
河北省教育厅高校强势特色学科资助项目[编号:冀教高(2005)52号]
河北省科技厅科技支撑课题(编号:0727611006)
