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Human transcription factor genes involved in neuronal development tend to have high GC content and CpG elements in the proximal promoter region

Human transcription factor genes involved in neuronal development tend to have high GC content and CpG elements in the proximal promoter region
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摘要 Transcription factors（TFs）play critical roles in the development of the nervous system,but the transcriptional regulatory mechanisms of these genes are poorly understood.Here we analyzed 5-kb of the 5＇ flanking genomic DNA sequences of 41 TF genes involved in neuronal development.The results showed that the TF genes tend to have higher GC contents in the proximal region and most of the TF genes have at least one proximal GC-rich（GC content60%）promoter with a CpG island.The promoter distribution analysis showed that the GC-poor promoters were sporadically distributed within the 5-kb flanking genomic sequence（FGS）;however,more than half（37 of 70）of the GC-rich promoters were located in the proximal region between nucleotides—1 and—500.Luciferase assays showed that partial GC-rich promoters increased gene expression in SH-SY5Y cells and that CpG methylation repressed the promoter activity.This study suggests a potential general mechanism for regulation of TF expression. Transcription factors（TFs）play critical roles in the development of the nervous system,but the transcriptional regulatory mechanisms of these genes are poorly understood.Here we analyzed 5-kb of the 5＇ flanking genomic DNA sequences of 41 TF genes involved in neuronal development.The results showed that the TF genes tend to have higher GC contents in the proximal region and most of the TF genes have at least one proximal GC-rich（GC content60%）promoter with a CpG island.The promoter distribution analysis showed that the GC-poor promoters were sporadically distributed within the 5-kb flanking genomic sequence（FGS）;however,more than half（37 of 70）of the GC-rich promoters were located in the proximal region between nucleotides—1 and—500.Luciferase assays showed that partial GC-rich promoters increased gene expression in SH-SY5Y cells and that CpG methylation repressed the promoter activity.This study suggests a potential general mechanism for regulation of TF expression.
作者 Yue-Sheng Long Jia-Ming Qin Tao Su Qi-Hua Zhao Yong-Hong Yi Wei-Ping Liao
机构地区 Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China
出处 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2011年第4期157-163,共7页 遗传学报（英文版）
基金 supported by the National Natural Science Foundation of China (Nos. 31070928, 30600198, 30870876 and 81000558) the Guangzhou Scholar Project (Nos. 10A011G and 10A012G) the Scientific Research of Guangzhou Municipal Colleges and Universities (No. 10A211).
关键词 Transcription factor PROMOTER GC content CpG island DNA methylation Transcription factor Promoter GC content CpG island DNA methylation
分类号 Q933 [生物学—微生物学] Q987 [生物学—遗传学]
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参考文献26

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Journal of Genetics and Genomics
2011年 第4期

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