摘要
目的:探讨补肾化瘀方对肾纤维化大鼠肾组织中α-平滑肌肌动蛋白(α-SMA)表达的影响。方法:将24只大鼠随机分为假手术组、模型组、补肾化瘀方组和代文组,采用单侧输尿管结扎(UUO)的方法建立肾纤维化大鼠模型;分别于UUO术后28天采用免疫组化方法观察模型大鼠肾组织α-SMA的表达变化。结果:与假手术组相比,模型组大鼠肾组织α-SMA表达于28天出现明显增高(P<0.01);用药后,补肾化瘀汤组和代文组α-SMA表达与模型组相比均明显降低(P<0.01);补肾化瘀汤组和代文组无明显差异(P>0.05)。结论:补肾化瘀汤组能够通过降低肾组织中α-SMA表达防治肾纤维化。
Objective：To investigate the influence of BuShen formula on the expression of α-Smooth Muscle Actin（α-SMA）in the Rat with Renal Interstitial Fibrosis.Methods：24 rats were randomly divided into control group,model group,Daiwen treatment group and BuShen formula treatment group.The rat models with Renal Interstitial Fibrosis were induced by unilateral ureteral obstruction.The expressions ofα-SMA in the rat kidneys were detected at 28th day by immunohistochemistry.Results： Compared to the controls,the expression of α-SMA was significantly increased in models（P0.01）.Compared to models,all the indicators mentioned above were improved after given drugs（P0.01）.BuShen formula treatment group is as good as Daiwen group（P0.05）.Conclusions： BuShen formula can prevent renal interstitial fibrosis through decreasing the expression of α-SMA.
出处
《中国民康医学》
2011年第9期1081-1082,1084,共3页
Medical Journal of Chinese People’s Health
