摘要
目的观察椒苯酮胺(piperphentonamine,PPTA)对大鼠脑缺血再灌注损伤后的保护作用,并探讨其作用机制。方法随机将SD大鼠分为假手术组、模型组、PPTA组(2.5,5,10 mg/kg)和阳性对照依达拉奉组(6 mg/kg)。采用大鼠大脑中动脉闭塞(MCAO)2 h再灌注模型,缺血1 h后静脉注射给药,复通灌流24 h后,采用Zea-Longa法进行神经功能缺陷评分,检测大鼠脑组织含水量,TTC染色法测定梗死体积及脑组织中SOD、MDA、GSH、NO、NOS生化指标。结果与模型组相比,PPTA组神经功能评分减低,梗死体积减少,脑组织中SOD、GSH活力增加,NOS活力降低,MDA和NO含量减少。其中以高剂量(10 mg/kg)组改变最为明显。结论 PPTA可能通过抑制脂质过氧反应和清除氧自由基等机制,发挥神经保护作用。
Objective To test the neuroprotective effect of piperphentonamine hydrochloride（PPTA） and investigate the potential underlying mechanisms in focal cerebral ischemia-reperfusion in rats.Methods Sprague-Dawley rats were randomly divided into the sham group,the ischemia-reperfusion group,the PPTA treated group（2.5,5,and 10 mg/kg） and the edaravone treated group（6 mg/kg）.Rats were subjected to 24 h of reperfusion following ischemia for 2 h induced by middle cerebral artery occlusion（MCAO）.The different doses of PPTA were intravenously administered to rats 1 h after the onset of ischemia.The neurological behavioral test was performed 24 h after reperfusion by using the Zea-Longa scores.Infarction volumes were calculated by TTC-staining.The changes in water content,and the activity of SOD,GSH,NOS and the content of MDA and NO in brain tissue were measured.Results Compared with the ischemia-reperfusion group,the treatment with PPTA improved Zea-Longa scores,reduced infarction volume and brain water content.PPTA treatment also increased activities of SOD and GSH,decreased NOS activity,and reduced the levels of MDA and NO.The effect in high-dose group（10 mg/kg） was the most obvious in the present study.Conclusion This study suggests that the neuroprotective effects of PPTA are closely associated with inhibition of lipid peroxidation reaction and scavenging free radicals.
出处
《军事医学》
CAS
CSCD
北大核心
2011年第4期286-289,共4页
Military Medical Sciences
基金
国家自然科学基金项目(30973518)
广东省自然科学基金重点项目(7117782)
