摘要
目的:研究肿瘤耐药相关基因P-gp和ABCG2在食管鳞状细胞癌组织中的表达及其临床病理特征和预后的关系.方法:应用免疫组织化学SP法检测食管鳞状细胞癌患者76例及正常食管黏膜30例,观察肿瘤耐药相关基因P-gp和ABCG2的表达,同时回顾性研究了这些表达与食管鳞状细胞癌发生、浸润及转移等临床病理学参数的关系.结果:(1)食管鳞状细胞癌组织中,P-gp和ABCG2的表达阳性率分别为88.16%和80.26%.30例食管正常黏膜中两者的表达率分别为60%和50%.差异均有统计学意义(P<0.05);(2)食管鳞状细胞癌组织中,P-gp在不同临床分期、不同浸润深度组之间阳性表达率的差异有统计学意义(P=0.002,0.035);(3)食管鳞状细胞癌组织中,ABCG2在不同临床分期、有或无淋巴结转移组之间阳性表达率的差异有统计学意义(P=0.006,0.034);(4)P-gp与ABCG2的表达呈正相关(r=0.228,P<0.05);(5)本组病例的5年总生存率为34.21%,单因素生存分析表明,P-gp阳性表达组的生存率显著低于阴性表达组,两组间生存率的差异有统计学意义(P<0.05).结论:P-gp和ABCG2在食管鳞癌的发生发展中可能起一定的作用.P-gp与食管鳞癌的预后密切相关.
AIM：To investigate the expression of P-glycoprotein（P-gp） and ATP-binding cassette superfamily G member 2（ABCG2） in esophageal squamous cell carcinoma（ESCC） and to analyze their clinicopathologic significance.METHODS：Immunohistochemistry was used to detect the expression of P-gp and ABCG2 in 76 cases of ESCC and in 30 cases of normal esophageal tissue.The relationship of P-gp and ABCG2 expression with clinicopathologic parameters of ESCC was then analyzed.RESULTS：The positive rates of P-gp and ABCG2 expression were 88.16% and 80.26% in ESCC and 60% and 50% in normal esophageal tissue,respectively,with statistically significant differences among the two groups（both P 0.05）.The expression of P-gp protein was signif icantly associated with tumor clinical stage and depth of invasion in ESCC,while that of ABCG2 was significantly associated with tumor clinical stage and lymph node metastasis.The expression of P-gp was positively correlated with that of ABCG2（r=0.228,P0.05）.The 5-year overall survival rate for all patients was 34.21%.Univariate survival analysis showed that the fiveyear survival rate for P-gp-positive patients was significantly lower than that for P-gp-negative ones（P0.05）.CONCLUSION：P-gp and ABCG2 play an important role in the pathogenesis of ESCC.The expression of P-gp is closely related to prognosis of ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第7期728-733,共6页
World Chinese Journal of Digestology
基金
新疆地方病分子生物学重点实验室开放课题基金资助项目No. XJDX0208-2006-09~~
