摘要
目的评价免疫表型在骨髓增生异常综合征（MDS）诊断中的价值。方法采用流式细胞术对27例MDS患者的骨髓细胞进行免疫表型检测。结果随着MDS疾病的进展，cD^＋34细胞比例逐渐升高，分别为：难治性贫血／环形铁粒幼细胞性难治性贫血（RA／RAS）7．43％，难治性贫血伴原始细胞增多（RAEB）36．81％，难治性贫血伴原始细胞增多转化型（RAEB-T）56．45％，3组差异有统计学意义（F=51．197，P=0．000），且各组间差异均有统计学意义（P〈0．05）；髓系抗原CD33、CD13HLA-DR表达逐渐增高，CD14、CD15，抗原表达随着疾病的进展逐渐降低，3组间差异有统计学意义（P〈0．05）；B淋巴细胞表面抗原CD19、CD10的表达随着疾病进展而降低；T淋巴细胞表面抗原CD7表达随着疾病进展而增高，分别为RA／RAS2．63％、RAEB10．79％和RAEB-T11．00％，3组间差异有统计学意义（F=10．439，P=0．001），其中RA／RAS组与RAEB组、RAEB-T组之间差异有统计学意义（P=0．000，P=0．001）。结论检测MDS患者骨髓细胞的免疫表型有助于MDS的诊断、分型和判断预后，从而为治疗提供依据。
Objective To evaluate the value of imnmnophenotype in diagnosis of myelodysplastic syndrome （MDS）. Methods The immunophenotype of bone marrow cells in 27 patients with MDS were detected by monoclonal antibody by flow cytometry. Results As the progression of the disease, CD34 positive cells gradually increased： refractory anemia/ring sideroblasts refractory anemia （RA/RAS） 7.43 %, refractory anemia with excess of blasts （RAEB） 36.81%, refractory anemia with excess of blasts transformed （RAEB-T） 56.45 %, and the differences were statistically significant （P 〈0.05）; the expressions of CD33, CD13 and HLA- DR increased gradually, the expressions of CD14 and CDt5 antigens gradually decreased, the difference of three groups was statistically significant （P 〈0.05）, the differences between RA/RAS and RAEB-T, RAEB and RAEB-T were statistically significant （P 〈0.05）; the expression of CD19 and CD10 decreased and the expression of CD7 increased （RA/RAS 2.63 %, RAEB 10.79 % and RAEB-T ll.O0 %） with the progression of the disease, the difference of three groups was statistically significant （F =10.439, P 〈0.05）, the differences between RA/RAS and RAEB, RA/RAS and RAEB-T were statistically significant （P 〈0.05）. Conclusion The detection of immunophenotype of bone marrow cells in patients with MDS contributes to the diagnosis, classification and prognosis of MDS.
出处
《肿瘤研究与临床》
CAS
2011年第4期256-258,共3页
Cancer Research and Clinic
