摘要
目的：研究抑癌基因ｐ１６ 与大肠癌发生发展的关系．方法：采用免疫组织化学 Ａ Ｂ Ｃ法检测了３１ 例结肠癌，２２ 例直肠癌，２８ 例正常大肠组织中抑癌基因ｐ１６ 的表达情况． 结果：正常大肠组织和大肠癌组织中ｐ１６ 蛋白的阳性率分别为 ７５％（２１／２８）和３５．７％ （１９／５３， Ｐ＜ ００１）；ｐ１６ 蛋白表达随着肿瘤组织分化程度降低而减弱，阳性表达率有显著性差异（ Ｐ＜００５）． 结论： ｐ１６ 蛋白表达缺失与肿瘤分化不良有关 （ ｒ＝－ ０．３３８３， Ｐ＜ ０．０５）。
AIM: To investigate the relationship between p 16 gene and the development and occurrence of colorectal cancer. METHODS:Using ABC immunohistochemistry methods, we investigated the expression of p 16 gene in 31 cases of colon cancer, 22 of rectal cancer and 28 of normal colorectal tissue. RESULTS:The positive staining rate was 75%(21/28) in normal colorectal tissue and 35.7%(19/53) in colorectal cancer( P <0.01). The expression of p 16 gene was reduced with the cancer′s malignant degree ( P <0.05). CONCLUSION:The mutation and loss of p 16 gene was closely related to the occurence and development of colorectal cancer ( r =-0.3383 P <0.05). p 16 gene plays an important role in the estimation of differential degree of cancer′s malignant and prognosis.
出处
《第四军医大学学报》
1999年第7期619-621,共3页
Journal of the Fourth Military Medical University
