摘要
目的：获得具有活性ａｎｇｉｏｓｔａｔｉｎ 的表达，为进一步开发应用奠定基础． 方法：用 Ｐ Ｃ Ｒ 方法从人纤维蛋白酶原基因中，获得ａｎｇｉｏｓｔａｔｉｎ（ Ｋ１３）的基因，用原核表达载体 ｐ Ｂ Ｖ２２０，表达非融合ａｎｇｉｏｓｔａｔｉｎ（ Ｋ１３）． 结果：得到有抑制活性ａｎｇｉｏｓｔａｔｉｎ（ Ｋ１３）的表达，但表达量较低． 结论：ａｎｇｉｏｓｔａｔｉｎ（ Ｋ１３）可在原核非融合蛋白表达载体中得到表达．
AIM: To acquire the clone and expression of angiostatin. METHODS: Angiostatin(K1 3) cDNA, acquired from plasminogen cDNA by PCR, was inserted into pBS vector to analysis its DNA sequence and was inserted into pBV220 vector to express angiostatin protein.RESULTS: Angiostatin(K1 3) cDNA was acquired and so was low level expression of angiostatin(K1 3). CONCLUSION:Active angiostatin( K1 3) can be reached using unfusion vector,pBV220/DH5α.
出处
《第四军医大学学报》
1999年第7期607-609,共3页
Journal of the Fourth Military Medical University
