摘要
目的通过建立RhD阴性供者与红细胞-80℃深低温保存信息库,分析本地区RhD阴性血型分布状况,合理制备和保存RhD阴性成分血。方法采集无偿献血者标本,用血型血清学方法筛选RhD阴性供者,并进行RhD阴性鉴定确认和CcE表型分型,创建RhD阴性供者信息库。按计划制备并用深低温等技术保存RhD阴性成分血。结果 1998年10月～2010年6月共筛查无偿献血者384334例,最后鉴定确认RhD阴性供者1126(2.93‰)例。其中A型200例(32.63%)、B型153例(24.96%)、O型203例(33.12%)和AB型57例(9.30%)。表型ccdee369(60.20%)例,Ccdee179(29.20%)例,CCdee38(6.20%)例,ccdE19(3.10%)例,CcdE8(1.31%)例。10年向临床供应RhD阴性血液1375单位。结论建立RhD阴性供者信息库,为输注RhD阴性血的患者,特别是已产生同种抗体患者,提供RhD和CcEe表型相配合的供者血液,有效预防因Rh抗原同种免疫反应所致的各类溶血性输血反应,提高血液输注的疗效。
Objective To establish a information database of RhD-negative and red cell -80℃ cryopreservation, analyze the distribution of RhD negative blood group in local area, then reasonably make and preserve componential blood of RhD-negative. Methods By collecting the blood samples of volunteer blood donors, screen RhD-negative donors with serological and identified for RhD-negative confirmation and CcE phenotype, create a RhD-negative donor database. Preparation according to plan and save with deep hypothermia and other technology components RhD negative blood. Results During October 1998 and June 2010,384 334 cases of voluntary blood donors were screened, the final and identified as RhD negative donors 1 126（2.93 ‰）. Of which 200 cases of type A （32.63%）, B-type of 153 cases （24. 96%）, O-type 203 cases （33. 12%） and AB type 57 cases （9.30%）. Phenotype ccdee 369 cases （60.20%） ,Ccdee 179 cases （29.20%）,CCdee 38 cases （6.20%）,ccdE 19 cases （3.10~） and CcdE 8 cases （1. 31%）. 10 years RhD-negative blood supply to the clinical 1 375 U. Conclusion RhD-negative donor information database, to RhD- negative blood transfusion patients, in particular, have produced the same kinds of antibodies were to provide RhD and CcEe phenotype matching of donor blood, effectively prevent Rh antigens the same kind of immune response to the various types of hemolytic transfusion reactions and improve the efficacy of blood transfusion.
出处
《国际检验医学杂志》
CAS
2011年第5期548-549,552,共3页
International Journal of Laboratory Medicine
关键词
RHD阴性
信息库
红细胞
深低温保存
临床应用
RhD negative, information database
red blood cell
Cryopreservation
clinical application
