摘要
将黄曲霉毒Ｂ１－牛血清白蛋白（ＡＦＢ１Ｏ—ＢＳＡ）连接物免疫注射４只（Ａ、Ｂ、Ｃ、Ｄ）产蛋鸡。研究了抗ＡＦＢ１蛋黄抗体的产生进程，除鸡Ｂ外，其它３只鸡从第一次注射抗原后的第９０天开始有较明显的抗体产生，第１３５天达到高峰，从第１６５天开始下降。在抗体产生高峰期，蛋黄抗体Ａ、Ｃ、Ｄ的间接ＥＬＩＳＡ效价分别为１∶８０００、１∶６０００、１∶６０００。以蛋黄抗体Ａ为材料，研究了抗体的特异性，结果表明：该抗体有较好的特异性。但是抗体与ＡＦＢ１的结构类似物（ＡＦＢ２、ＡＦＧ２、ＡＦＧ１、ＡＦＭ１）除ＡＦＭ１外都有不同程度的交叉反应，达到５０％的竞争抑制率时，ＡＦＢ１、ＡＦＢ２、ＡＦＧ１、ＡＦＧ２的灵敏度分别为６、２５、１２５和２４９５ｎｇ／ｍｌ。
AFB 1O BSA conjugates were injected into four laying hens(A,B,C and D).The yolk anti AFB 1 antibodies A,C and D produced were obviously increased after 90 days of the first injection,and reached the peak after 135 days,and started dropping after 165 days.The indirect ELISA titer of yolk antibodies A,C and D were 1∶8000,1∶6000 and 1∶6000 respectively.The specificity of the antibody A was good, but a cross reaction with AFB 2,AFG 1,AFG 2, with an exception of AFM 1,was found.The sensitivities of AFB 1,AFB 2,AFG 1 and AFG 2 were 6,25,125 and 2495 μg/L respectively.
出处
《卫生研究》
CAS
CSCD
北大核心
1999年第4期241-243,共3页
Journal of Hygiene Research
基金
湖北省科学技术委员会重点课题
