摘要
本研究建立了用于检测粮食、饲料中伏马菌素Ｂ１（ＦＢ１）的快速、灵敏、简便的免疫检测方法—建立在单克隆抗体基础上的直接竞争性酶联免疫吸附测定方法（ＤＣ—ＥＬＩＳＡ法）；其最低检出浓度为１０ｎｇ／ｍｌ，线性范围在１０ｎｇ～５μｇ／ｍｌ。对新疆、安徽、黑龙江、哈尔滨四省市玉米样品中的ＦＢ１含量进行了测定，结果表明１５６份样品中有３４份检出ＦＢ１，阳性率为２１．７９％，含量在０．１８～３１．３２μｇ／ｇ范围内，平均含量为１２．０４μｇ／ｇ。
Direct competitive inhibition enzyme linked immunosorbent assay for determination of fumonisin B 1(FB 1) in corns were developed by using anti FB 1 monoclonal antibody.The minimum detectable concentration of FB 1 was 10ng/ml,and the standard curve was linear between 10ng/ml and 5μg/ml The FB 1 concentration of some corn samples from Xinjiang,Anhui,Hilongjiang provinces and Haerbin city were detected.The result imply that 34 out of 156 samples were positive for FB 1 .The concentrations were in the range of 0 18～31 32μg/g.The average concentration was 12 04μg/g
出处
《卫生研究》
CAS
CSCD
北大核心
1999年第4期238-240,共3页
Journal of Hygiene Research
基金
国家自然科学青年基金
