摘要
目的：建立一种新缺氧动物模型，为保健品抗缺氧实验提供新筛选方法。方法：选用初成年雄性昆明种小鼠，随机分为对照组和缺氧组。对照组动物在常压环境条件下喂养，缺氧组动物在常压缺氧舱中喂养。舱内通入缺氧气体（氮：空气为1：1的混合气，氧浓度为10％±1％），连续20天。分别于缺氧5天、10天、15天、20天分批处死缺氧组动物，第20无处死对照组动物。取动物肺组织作病理检查。选择计数小于100微米肌性血管（MA）、部分肌性血管（PMA）、非肌性血管（NMA）的肺血管数及三者的构成比。结果：随缺氧时间延长，缺氧组MA、PMA的比例较对照组明显增大（P＜0．01），而NMA的比例较对照组明显减少。缺氧15天和20天三种血管的比例越于稳定。结论：在氧气浓度为10％的环境中连续缺氧15天至20天可形成较为理想的慢性缺氧性小鼠肺血管结构重建模型。
Objective: To construet the model of pulmonarp vascular structure remodeling PVSR of chronic hypoxic mice. Motheds: 50 matured male Kunming mice were divided randomly into the eontrol group and hypoxic group. The former was fed in normal condition, while the latter was fed the hypoxic group were fed in the normobaric hypoxic chamber, in which a mixture of gases(N2: Air=1:1) were ventilated in ouler that the O2 concentration was reduced to 10%±1 %. The mice were killed after 5, 10, 15 and 20 days of hypoxia. At 20th day the control group mice were also killed.Pulmonary tissue was then taken out to examine the patholopical change. MA, PMA and NMA of pulmonary vases (<100μm) were calculated. Results: The ratio of MA and PMA increased, but the ratio of NMA decreased significantly with the hypoxic time in hypoic groups compared with the control group (P<0.01). But the difference of the three ratios of vases between 15 and 20 days of hypoxic group was not significant. Conclusions: Hypoxia for 15~20 days in 10% O2 concentration environment, the model of the PVSR of chronic hypoxic mice could be constructed.
出处
《泸州医学院学报》
1999年第3期197-199,共3页
Journal of Luzhou Medical College
关键词
缺氧
肺血管结构重建
动物模型
肺动脉高压
Hypoxia
Pulmonary vascular structure remodeling
Animal model
Mice
