摘要
目的：探讨氧化高密度脂蛋白3（ox-HDL3）对人脐静脉内皮细胞（HUVECs）的损伤作用。方法：体外培养HU-VECs与不同浓度（50~200mg/L）ox-HDL3共孵育,用普通光镜和荧光显微镜分别观察HUVECs形态及核形态的变化,用噻唑兰（MTT）比色法检测细胞存活状况。结果：ox-HDL3使内皮细胞数目减少,形态改变;Hoechst 33258染色后可见大量的细胞核浓缩呈高亮度蓝色荧光的凋亡细胞;MTT结果显示,细胞存活率下降,且呈剂量依赖性。结论：ox-HDL3可导致内皮细胞受损,从而促进动脉粥样硬化的发生发展。
Objective：To explore the effect of ox-HDL3 on human umbilical vein endothelial cell（HUVEC）.Methods：HUVECs were incubated with ox-HDL3（50、100、200mg/L） for 24 hours.The change of cell shape and nuclear morphology were observed by light microscope and nuclear staining with Hoechst 33258.The cells survival percentage was detected by MTT assay.Results：Compared with the control group,the number of HUVECs cells incubated with ox-HDL3 decreased and the shape changed.Cells appeared typical characteristics of apoptosis,including apoptotic nuclear condensation.The survival rate of cells was reduced by MTT assay.Conclusion：ox-HDL3 can lead to HUVECs damage.
出处
《医学理论与实践》
2011年第7期747-749,共3页
The Journal of Medical Theory and Practice
