摘要
分别采用热水浸提法、超声波辅助提取法两种工艺提取板栗多糖,经乙醇沉淀、Sevage法除蛋白、有机溶剂脱脂后用蒽酮-硫酸比色法测定其含量,通过单因素试验和正交试验确定不同工艺的最佳提取工艺参数。结果表明:1 g板粟粉,热水浸提法提取板栗多糖的最佳条件为提取温度60℃,提取时间1.5 h,液料比20∶1(mL∶g),板栗多糖得率为9.85%;超声波辅助提取法的最佳提取条件为提取温度65℃、液料比35∶1(mL∶g)、提取时间45 min、超声波功率160 W,在最佳工艺条件下板栗多糖得率达到10.67%。
Polysaccharides were extracted from chestnut using hot water extraction and ultrasound-assisted extraction.The contents of chestnut polysaccharides extracted by the two techniques were determined by anthrone-H2SO4 method after ethanol precipitation,Sevage deproteinization and organic solvent degreasing.The optimal extraction conditions were obtained by single factor and orthogonal experiments.The results showed that,the optimal conditions of hot water extraction were： chestnut 1 g,extraction temperature 60 ℃,liquid-solid ratio 20∶1（mL∶g）,extraction time 1.5 h,the yield of polysaccharides was 9.85 %;The optimal conditions of ultrasonic extraction were： extraction temperature 65 ℃,liquid-solid ratio 35∶1（mL∶g）,extraction time 45 min,ultrasonic power 160 W,the yield of polysaccharides was 10.67 %.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2011年第2期96-100,共5页
Chemistry and Industry of Forest Products
基金
农业科技成果转化资金项目(2008GB2A200014)
河北省科技支撑计划项目(09231001D-1)
关键词
板栗
多糖
热水浸提法
超声波辅助浸提法
chestnut
polysaccharide
hot water extraction
ultrasound-assisted extraction
