摘要
为评价表达致龋变异链球菌表面蛋白和霍乱毒素B亚单位的转基因番茄防龋疫苗的安全性,进行了大鼠经口灌胃染毒试验。结果表明,急性毒性试验中大鼠均未见毒性症状,急性毒性LD50>20 g/kg;亚慢性毒性试验中大鼠均未见毒性症状,在血液学和生化、脏器系数和组织病理学检查中,试验组大鼠与阴性对照组间均无显著差异(P>0.05)。说明,表达致龋变异链球菌表面蛋白和霍乱毒素B亚单位嵌合体蛋白的转基因番茄防龋疫苗对大鼠安全。
The healthy rats were perfused with transgenic tomato juice containing PAcA/CTB protein and PAcP/CTB protein to evaluate the safety of transgenic tomato anti-caries vaccine with surface protein of streptococcus mutans and cholera toxin B subunit.The results showed that the toxic symptom was not found on tested rats in the acute and subchronic toxicity tests,the LD50 of acute toxicity was more than 20g/kg,there were no significant difference in hematological index,blood biochemistry index,organ coefficient and histopathology between the tested group and negative CK group,which indicates that transgenic tomato anti-caries vaccine with surface protein of streptococcus mutans and cholera toxin B subunit is safe to rats.
出处
《贵州农业科学》
CAS
北大核心
2011年第4期146-149,共4页
Guizhou Agricultural Sciences
基金
国家自然基金资助项目"转基因植物可食融合体防龋疫苗的研究"(30160086)
贵州省优秀科技人才省长专项基金资助项目"转基因植物防龋疫苗生态安全性的初步研究"[黔省专合字(2006)44]
贵州省科技攻关项目"转基因植物防龋疫苗的免疫效果和安全性研究"[黔科合NY字(2006)3043]
贵阳市科学技术计划项目"嵌合体转基因植物防龋疫苗的免疫原性和安全性研究"[2008筑科农合同字第28-6号]
关键词
转基因番茄
防龋疫苗
急性毒性
亚慢性毒性
龋病
变异链球菌
transgenic tomato
anti-caries vaccine
acute toxicity
subchronic toxicity
dental caries
streptococcus mutans
