摘要
从广州石化厂曝气池周围的油泥中筛选驯化出2株可以降解稠油的菌株GS02和GS07,经16S rDNA序列分析鉴定这两株菌均属于不动杆菌属（Acinetobacter sp.）。通过摇瓶振荡实验,对GS02和GS07在以稠油为唯一碳源时的生长情况、降解率及环境条件对降解率的影响进行了研究。结果表明,菌株GS02和GS07对1 g/L的稠油5 d的降解率为45.3%和46.1%。另外,这2株菌对稠油的降解率随初始稠油浓度的升高而降低,最佳降解温度为28℃和30℃,最佳pH值为7.2和7.5,最适盐浓度为4~6 g/L和4 g/L。将残余稠油萃取后用GC-MS分析,发现GS02和GS07对烷烃及生标化合物植烷和姥鲛烷都有很好的去除效果。
Two bacterial strains GS02 and GS07 degrading heavy oil were enriched and screened from oil sludge collected from Sinopec Guangzhou Company.Both of the two strains were identified as Acinetobacter sp.by using 16S rDNA sequencing.Preliminary studies of the bacterial growth,the degradation rate as well as the effect of environmental parameters on the heavy oil degradation were conducted in shaking flasks using heavy oil as the sole carbon source.The degradation rate of 1g/L heavy oil was 45.3% and 46.1% by GS02 and GS07 after 5 days,respectively.The degradation rates of the heavy oil reduced with the increasing of the initial heavy oil concentration.The optimum degradation temperatures were 28 ℃ and 30 ℃,the optimum pH were 7.0 and 7.2,and the optimum salt concentrations were 4~6 g/L and 4 g/L,respectively.The residual heavy oil was analyzed by gas chromatography-mass spectrometry（GC-MS） and results showed that both strains GS02 and GS07 had good ability to remove alkanes as well as biomarker compounds such as phytane and pristine.
出处
《环境工程学报》
CAS
CSCD
北大核心
2011年第5期1181-1186,共6页
Chinese Journal of Environmental Engineering
基金
广东省科技计划项目(2007A050100023)
广东省自然科学基金资助项目(9351064101000001)
广州市环保局科技成果应用示范项目(研发2009年No.01)
关键词
稠油
不动杆菌
生物降解
影响因素
heavy oil
Acinetobacter sp.
biodegradation
influencing factors
