摘要
目的阐明性成熟前食蟹猴生精细胞的发育进程。方法分别采集性成熟前不同年龄(0岁、0.5岁、1岁、1.5岁、2岁、2.5岁、3岁、3.5岁、4岁)食蟹猴睾丸,制作石蜡切片,进行HE染色和PAS/H染色。根据生精细胞的染色特性,分析性成熟前食蟹猴生精细胞的发育进程,并对食蟹猴精原干细胞进行初步鉴定。结果 HE染色结果显示,1岁及以下食蟹猴生精上皮上生精细胞仅有精原干细胞(包括Ad、At及Ap型精原细胞),1.5岁食蟹猴生精上皮上开始出现B型精原细胞,3岁食蟹猴生精上皮上出现精母细胞,4岁食蟹猴生精上皮上出现从精原干细胞到精子的所有生殖细胞。PAS/H染色结果显示,1～2.5岁食蟹猴Ad型精原细胞胞质呈PAS阳性,At型精原细胞胞质呈PAS弱阳性,Ap型精原细胞胞质呈PAS阴性;其他生精细胞及支持细胞胞质呈阴性;0.5岁及以下,3岁及以上食蟹猴生精细胞的胞质PAS/H染色特性与前者存在差异。结论本文详细阐述了性成熟前食蟹猴生精细胞随年龄增长的渐次性发育模式,并建立了性成熟前食蟹猴精原干细胞原位鉴定的一种新方法,这些研究结果为食蟹猴精原干细胞的其他相关研究奠定了基础。
Objective To elucidate the developmental process of spermatogenic cells in immature Cynomolgus monkey.Method Testis of 0,0.5,1,1.5,2,2.5,3,3.5,4 years old Cynomolgus monkeys were collected,paraffin sections were made,stained with HE and PAS /H（pexiodic acid-schiff /hematoxylin,PAS /H）.Based on the staining pattern,the developental process of spermatogenic cells in immature Cynomolgus monkey was analysed and spermatogonial stem cells were primarily identified.Result HE Staining showed spermato gonial stem cells（including dark type A spermatogonia,transitional type A spermatogonia and pale type A spermatogonia） were the only spermatogenic cells in seminiferous epithelium of Cynomolgus monkey at 1 year old and below,Type B spermatogonia emerged at 1.5 years old,spermatocyte emerged at 3 years old,and all types of spermatogenic cells（from spermatogonial stem cells to sperm） were present at 4 years old Cynomolgus monkey.PAS /H staining showed cytoplast of dark type A spermatogonia as positive,transitional type A spermatogonia as weakly positive,and pale type A spermatogonia as negative,while cytoplast of sertoli cells and other spermato genic cells as negative in Cynomolgus monkey between one year old and 2.5 years old.Cytoplast of spermatogenic cells have a different PAS /H staining pattern in Cynomolgus monkey at 6 months old and below,3 years old and above.Conclusion This article detailedly elucidated the progressive development model of spermatogenic cells with age and established a new method for in situ identification spermatogonial stem cells in immature Cynomolgus monkey.
出处
《中国比较医学杂志》
CAS
2011年第3期31-35,82,共6页
Chinese Journal of Comparative Medicine
基金
广东省科学院基金(styz2008)
关键词
食蟹猴
生精细胞
发育进程
Cynomolgus monkey
Spermatogonic cells
Developmental process
