摘要
目的:研究紫草色素对兔耳增生性瘢痕的抑制作用,并对其作用机制进行初步探讨。方法:建立兔耳增生性瘢痕动物模型,造模成功后分为模型对照组(M组)和紫草色素组(S组)。肉眼观察紫草色素对兔耳增生性瘢痕组织学影响,采用免疫组织化学法对样本进行成纤维细胞计数、CD34+血管计数及对TGF-β1、TNF-α进行阳性表达,比较S组与M组在组织形态学、成纤维细胞数量与血管密度、TGF-β1、TNF-α阳性表达的差异。结果:组织形态学观察和兔耳瘢痕厚度测量结果显示,S组较M组能减淡瘢痕且降低瘢痕厚度;染色结果显示给予紫草色素后能够减少成纤维细胞数量和抑制血管增生,抑制TGF-β1及促进TNF-α阳性表达,两组比较均具有统计学意义(P<0.05,0.01)。结论:紫草色素能够抑制增生性瘢痕,且其机制可能与抑制成纤维细胞增殖、血管增生及TGF-β1的阳性表达,提高TNF-α阳性表达有关。
Objective： To study the inhibitory effect of shikonin on hypertrophic scar of rabbit ears,and discussed its possible mechanism.Methods： After the rabbit animal model of hypertrophic scar（HS） was successfully divided into model group（M group） and shikonin group（S group）.Observing the HS ears,and compared the of differences S group and M group in Fibroblasts,vascular density,TGF-β1 and TNF-α expression by immunohistochemistry.Results： The final results showed that S group was ketter than M group to fade scars and reduce scar thickness,the shikonin able to reduce the number of fibroblasts and the inhibition of vascular proliferation,inhibition of TGF-β1 and TNF-α expression,and the two groups were statistically significant（P0.05,0.01）.Conclusion： Shikonin can inhibit hypertrophic scars,which may be inhibite fibroblast proliferation,vascular proliferation and positive expression of TGF-β1,promote the positive expression of TNF-α.
出处
《四川中医》
2011年第4期54-58,共5页
Journal of Sichuan of Traditional Chinese Medicine
基金
国家自然科学基金地区科学项目:紫草色素抑制增生性瘢痕组织增生的机制研究(30660236)
